TY - JOUR
T1 - Structure and biochemical characterization of proliferating cellular nuclear antigen from a parasitic protozoon
AU - Cardona-Felix, Cesar S.
AU - Lara-Gonzalez, Samuel
AU - Brieba, Luis G.
PY - 2011/6
Y1 - 2011/6
N2 - Proliferating cellular nuclear antigen (PCNA) is a toroidal-shaped protein that is involved in cell-cycle control, DNA replication and DNA repair. Parasitic protozoa are early-diverged eukaryotes that are responsible for neglected diseases. In this work, a PCNA from a parasitic protozoon was identified, cloned and biochemically characterized and its crystal structure was determined. Structural and bio-chemical studies demonstrate that PCNA from Entamoeba histolytica assembles as a homotrimer that is able to interact with and stimulate the activity of a PCNA-interacting peptide-motif protein from E. histolytica, EhDNAligI. The data indicate a conservation of the biochemical mechanisms of PCNA-mediated interactions between metazoa, yeast and parasitic protozoa.
AB - Proliferating cellular nuclear antigen (PCNA) is a toroidal-shaped protein that is involved in cell-cycle control, DNA replication and DNA repair. Parasitic protozoa are early-diverged eukaryotes that are responsible for neglected diseases. In this work, a PCNA from a parasitic protozoon was identified, cloned and biochemically characterized and its crystal structure was determined. Structural and bio-chemical studies demonstrate that PCNA from Entamoeba histolytica assembles as a homotrimer that is able to interact with and stimulate the activity of a PCNA-interacting peptide-motif protein from E. histolytica, EhDNAligI. The data indicate a conservation of the biochemical mechanisms of PCNA-mediated interactions between metazoa, yeast and parasitic protozoa.
KW - Entamoeba histolytica
KW - proliferating cellular nuclear antigen
UR - http://www.scopus.com/inward/record.url?scp=79957979467&partnerID=8YFLogxK
U2 - 10.1107/S0907444911010547
DO - 10.1107/S0907444911010547
M3 - Artículo
SN - 0907-4449
VL - 67
SP - 497
EP - 505
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 6
ER -