Stable transformation of barley tissue culture by particle bombardment

A. Ritala; L. Mannonen; K. Aspegren; M. Salmenkallio-Marttila; U. Kurtén; R. Hannus; J. Mendez Lozano; T. H. Teeri; V. Kauppinen

doi:10.1007/BF00234708

Stable transformation of barley tissue culture by particle bombardment

A. Ritala, L. Mannonen, K. Aspegren, M. Salmenkallio-Marttila, U. Kurtén, R. Hannus, J. Mendez Lozano, T. H. Teeri, V. Kauppinen

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic Geneticin^R. Enzymatic activity was tested in the Geneticin^R resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

Original language	English
Pages (from-to)	435-440
Number of pages	6
Journal	Plant Cell Reports
Volume	12
Issue number	7-8
DOIs	https://doi.org/10.1007/BF00234708
State	Published - May 1993
Externally published	Yes

Access to Document

10.1007/BF00234708

Cite this

@article{762750fd40894e8a92bddb7e0a1bf547,

title = "Stable transformation of barley tissue culture by particle bombardment",

abstract = "Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.",

author = "A. Ritala and L. Mannonen and K. Aspegren and M. Salmenkallio-Marttila and U. Kurt{\'e}n and R. Hannus and {Mendez Lozano}, J. and Teeri, {T. H.} and V. Kauppinen",

year = "1993",

month = may,

doi = "10.1007/BF00234708",

language = "Ingl{\'e}s",

volume = "12",

pages = "435--440",

journal = "Plant Cell Reports",

issn = "0721-7714",

number = "7-8",

}

TY - JOUR

T1 - Stable transformation of barley tissue culture by particle bombardment

AU - Ritala, A.

AU - Mannonen, L.

AU - Aspegren, K.

AU - Salmenkallio-Marttila, M.

AU - Kurtén, U.

AU - Hannus, R.

AU - Mendez Lozano, J.

AU - Teeri, T. H.

AU - Kauppinen, V.

PY - 1993/5

Y1 - 1993/5

N2 - Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

AB - Suspension culture cells of barley (Hordeum vulgare L. cv Pokko) were stably transformed with two separate plasmids containing genes coding for neomycin phosphotransferase II and β-glucuronidase, respectively. Transformed cultures were selected with the antibiotic GeneticinR. Enzymatic activity was tested in the GeneticinR resistant cultures, and in 96% of them neomycin phosphotransferase could be detected. The non-selected marker, detected as β-glucuronidase activity, was expressed in 40% of the resistant cultures. Stable transformation was confirmed with Southern blot hybridization.

UR - http://www.scopus.com/inward/record.url?scp=0000903664&partnerID=8YFLogxK

U2 - 10.1007/BF00234708

DO - 10.1007/BF00234708

M3 - Artículo

C2 - 24197348

SN - 0721-7714

VL - 12

SP - 435

EP - 440

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 7-8

ER -

Stable transformation of barley tissue culture by particle bombardment

Abstract

Access to Document

Other files and links

Fingerprint

Cite this