TY - JOUR
T1 - Sister-chromatid exchange analysis in vivo using different 5-bromo-2′-deoxyuridine-labeling systems
AU - Madrigal-Bujaidar, E.
AU - Sánchez-Sánchez, M. A.
N1 - Funding Information:
E. Madrigal-Bujaidar was partially supported by COFAA. The authors are deeply grateful to Drs. Juan E. Rosas, Sandra Diaz Barriga A., Guadalupe Rivas, Silvia Aguirre, Arturo Pifia and RaM Quezada for their collaboration.
PY - 1991/1
Y1 - 1991/1
N2 - The quality of sister-chromatid differentiation, the basal rate of sister-chromatid exchanges (SCE) and the rate of cellular proliferation were studied in untreated and mitomycin C(MMC)-treated mice, using 4 different systems for administering 5-bromodeoxyuridine (BrdU): BrdU adsorbed to charcoal, tablets of BrdU mixed with cholesterol, tablets of BrdU coated with agar and tablets of BrdU partially coated with paraffin. The quality of sister-chromatid differentiation with the studied methods showed a useful stain contrast in an average of 75.4% second-division mitosas, with the lowest average occurring in mice implanted with agarcoated tablets. The frequency of SCE and the replicative index were similar in mice administered BrdU by all 4 systems both in control and in MMC-treated mice. From a practical point of view, the charcoal method could be done the fastest.
AB - The quality of sister-chromatid differentiation, the basal rate of sister-chromatid exchanges (SCE) and the rate of cellular proliferation were studied in untreated and mitomycin C(MMC)-treated mice, using 4 different systems for administering 5-bromodeoxyuridine (BrdU): BrdU adsorbed to charcoal, tablets of BrdU mixed with cholesterol, tablets of BrdU coated with agar and tablets of BrdU partially coated with paraffin. The quality of sister-chromatid differentiation with the studied methods showed a useful stain contrast in an average of 75.4% second-division mitosas, with the lowest average occurring in mice implanted with agarcoated tablets. The frequency of SCE and the replicative index were similar in mice administered BrdU by all 4 systems both in control and in MMC-treated mice. From a practical point of view, the charcoal method could be done the fastest.
KW - (Mouse)
KW - 5-Bromodeoxyuridine labeling
KW - Cholesterol
KW - Mitomycin C
KW - Sister-chromatid exchange
UR - http://www.scopus.com/inward/record.url?scp=0026088160&partnerID=8YFLogxK
U2 - 10.1016/0165-7992(91)90099-P
DO - 10.1016/0165-7992(91)90099-P
M3 - Artículo
SN - 0165-7992
VL - 262
SP - 15
EP - 19
JO - Mutation Research Letters
JF - Mutation Research Letters
IS - 1
ER -