TY - JOUR
T1 - Single multiplex polymerase chain reaction to detect diverse loci associated with diarrheagenic Escherichia coli
AU - López-Saucedo, Catalina
AU - Cerna, Jorge F.
AU - Villegas-Sepulveda, Nicolas
AU - Thompson, Rocio
AU - Velazquez, F. Raul
AU - Torres, Javier
AU - Tarr, Phillip I.
AU - Estrada-Garcia, Teresa
PY - 2003/1/1
Y1 - 2003/1/1
N2 - We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.
AB - We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga toxin-producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates in stool and food. Because of its simplicity, economy, and efficiency, this protocol warrants further evaluation in large, prospective studies of polymicrobial substances.
UR - http://www.scopus.com/inward/record.url?scp=0037240184&partnerID=8YFLogxK
U2 - 10.3201/eid0901.010507
DO - 10.3201/eid0901.010507
M3 - Artículo
SN - 1080-6040
VL - 9
SP - 127
EP - 131
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 1
ER -