TY - JOUR
T1 - Short- and long-term treatment with estradiol or progesterone modifies the expression of GFAP, MAP2 and Tau in prefrontal cortex and hippocampus
AU - Camacho-Arroyo, Ignacio
AU - González-Arenas, Aliesha
AU - Espinosa-Raya, Judith
AU - Piña-Medina, Ana Gabriela
AU - Picazo, Ofir
N1 - Funding Information:
We acknowledge the support for this study to SIP-IPN and COFAA as well as PAPIIT grant IN-201908 , DGAPA , UNAM . Authors thank Mr. Allan Larsen for checking the English language.
PY - 2011/7/18
Y1 - 2011/7/18
N2 - Aims: We analyzed the effects of the short- and long-term administration of estradiol (E2) or progesterone (P4) after ovariectomy on the expression of MAP2, Tau and GFAP in prefrontal cortex and hippocampus. Main methods: Sprague Dawley rats were ovariectomized and immediately treated with E2 or P4 for 2 or 18 weeks. At the end of treatments, hippocampus and prefrontal cortex were excised, proteins were extracted and MAP2, Tau and GFAP were analyzed by Western blot. Key findings: MAP2 and Tau content was not modified by E2 in the prefrontal cortex. On the contrary, P4 decreased MAP2 content after a short-term treatment, while it increased that of MAP2 and TAU in this brain region after a long-term treatment. E2 increased MAP2 content in hippocampus. In this region, short-term administration of P4 increased that of MAP2. GFAP content was diminished after a long-term administration of P4 in hippocampus. Significance: Current data emphasize the importance of short- and long-term sex steroid treatment on neuronal and glial cytoskeletal proteins expression.
AB - Aims: We analyzed the effects of the short- and long-term administration of estradiol (E2) or progesterone (P4) after ovariectomy on the expression of MAP2, Tau and GFAP in prefrontal cortex and hippocampus. Main methods: Sprague Dawley rats were ovariectomized and immediately treated with E2 or P4 for 2 or 18 weeks. At the end of treatments, hippocampus and prefrontal cortex were excised, proteins were extracted and MAP2, Tau and GFAP were analyzed by Western blot. Key findings: MAP2 and Tau content was not modified by E2 in the prefrontal cortex. On the contrary, P4 decreased MAP2 content after a short-term treatment, while it increased that of MAP2 and TAU in this brain region after a long-term treatment. E2 increased MAP2 content in hippocampus. In this region, short-term administration of P4 increased that of MAP2. GFAP content was diminished after a long-term administration of P4 in hippocampus. Significance: Current data emphasize the importance of short- and long-term sex steroid treatment on neuronal and glial cytoskeletal proteins expression.
KW - Estradiol
KW - GFAP
KW - MAP2
KW - Ovariectomy
KW - Progesterone
KW - Tau
UR - http://www.scopus.com/inward/record.url?scp=79959982606&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2011.05.008
DO - 10.1016/j.lfs.2011.05.008
M3 - Artículo
C2 - 21683086
SN - 0024-3205
VL - 89
SP - 123
EP - 128
JO - Life Sciences
JF - Life Sciences
IS - 3-4
ER -