TY - JOUR
T1 - Shear rate as scale-up criterion of the protein production with enhanced proteolytic activity by phosphate addition in the Jacaratia mexicana cell culture
AU - García-Salas, Sergio
AU - Gómez-Montes, Elías Octavio
AU - Ramírez-Sotelo, María Guadalupe
AU - Oliver-Salvador, María del Carmen
N1 - Publisher Copyright:
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - The main challenge in bioreactor scale-up is maintaining the bioprocess productivity. The aim of the present work was to scale-up the protein production with enhanced proteolytic activity by phosphate addition in Jacaratia mexicana cell culture, by using the shear rate as scale-up criterion. The volumetric proteolytic activity in the J. mexicana cell culture in shake flask increased 1.9 times with increasing phosphate concentration from 1.25 to 2.5 mmol L−1, while the protein concentration was similar (∼55 mg L−1). This culture was scaled-up from 0.4-L to 4-L in stirred tank bioreactors at average shear rate of 200 s−1 and 234 s−1, respectively, increasing the phosphate concentration in the 4-L bioreactor (2.5 mmol L−1) with respect to the 0.4-L bioreactor (1.25 mmol L−1). There was also an increase in the volumetric proteolytic activity (2 times), without statistically significant difference in the protein concentration. Scale-up strategy and improvement of the culture medium exhibited a similar behaviour maintaining the protein productivity and increasing the proteolytic activity. These results indicate that the average shear rate is a useful hydrodynamic parameter in the scale-up of protein production of J. mexicana cell culture. Additional phosphate and bioreactor hydrodynamics could be related to unusual lengthening and thinning of most cells in the 4-L stirred tank bioreactor. Finally, the power input per unit volume in the scaled-up bioreactor decreased by 53%, improving the volumetric proteolytic activity and maintaining protein productivity. These results encourage proceeding with the scale-up process using the shear rate as a scale-up criterion.
AB - The main challenge in bioreactor scale-up is maintaining the bioprocess productivity. The aim of the present work was to scale-up the protein production with enhanced proteolytic activity by phosphate addition in Jacaratia mexicana cell culture, by using the shear rate as scale-up criterion. The volumetric proteolytic activity in the J. mexicana cell culture in shake flask increased 1.9 times with increasing phosphate concentration from 1.25 to 2.5 mmol L−1, while the protein concentration was similar (∼55 mg L−1). This culture was scaled-up from 0.4-L to 4-L in stirred tank bioreactors at average shear rate of 200 s−1 and 234 s−1, respectively, increasing the phosphate concentration in the 4-L bioreactor (2.5 mmol L−1) with respect to the 0.4-L bioreactor (1.25 mmol L−1). There was also an increase in the volumetric proteolytic activity (2 times), without statistically significant difference in the protein concentration. Scale-up strategy and improvement of the culture medium exhibited a similar behaviour maintaining the protein productivity and increasing the proteolytic activity. These results indicate that the average shear rate is a useful hydrodynamic parameter in the scale-up of protein production of J. mexicana cell culture. Additional phosphate and bioreactor hydrodynamics could be related to unusual lengthening and thinning of most cells in the 4-L stirred tank bioreactor. Finally, the power input per unit volume in the scaled-up bioreactor decreased by 53%, improving the volumetric proteolytic activity and maintaining protein productivity. These results encourage proceeding with the scale-up process using the shear rate as a scale-up criterion.
KW - Stirred tank bioreactor
KW - average shear rate
KW - hydrodynamic parameters
KW - plant cell culture
KW - protein production
KW - proteolytic activity
UR - http://www.scopus.com/inward/record.url?scp=85111771194&partnerID=8YFLogxK
U2 - 10.1080/13102818.2021.1944317
DO - 10.1080/13102818.2021.1944317
M3 - Artículo
AN - SCOPUS:85111771194
SN - 1310-2818
VL - 35
SP - 1031
EP - 1042
JO - Biotechnology and Biotechnological Equipment
JF - Biotechnology and Biotechnological Equipment
IS - 1
ER -