TY - JOUR
T1 - Selection and identification of fungi isolated from sugarcane bagasse and their application for phenanthrene removal from soil
AU - Cortés-Espinosa, D. V.
AU - Fernández-Perrino, F. J.
AU - Arana-Cuenca, A.
AU - Esparza-García, F.
AU - Loera, O.
AU - Rodríguez-Vázquez, R.
PY - 2006/3
Y1 - 2006/3
N2 - This work investigated the identification and selection of fungi isolated from sugarcane bagasse and their application for phenanthrene (Phe) removal from soil. Fungi were identified by PCR amplification of ITS regions as Aspergillus terrus, Aspergillus fumigatus and Aspergillus niger, Penicillium glabrum and Cladosporium cladosporioides. A primary selection of fungi was accomplished in plate, considering Phe tolerance of every strain in two different media: potato dextrose agar (PDA) and mineral medium (MM). The radial extension rate (r r) in PDA exhibited significant differences (p < 0.05) at 200 and 400 ppm of Phe. A secondary selection of A. niger, C. cladosporoides, and P. glabrum sp. was achieved based on their tolerance to 200, 400, 600 and 800 ppm of Phe, in solid culture at a sugarcane bagasse/contaminated soil ratio of 95:5, in Toyamas, Czapeck and Wunder media. Under these conditions, a maximum (70%) Phe removal by A. niger was obtained. In addition C. cladosporioides and A. niger were able to remove high (800 ppm) Phe concentrations.
AB - This work investigated the identification and selection of fungi isolated from sugarcane bagasse and their application for phenanthrene (Phe) removal from soil. Fungi were identified by PCR amplification of ITS regions as Aspergillus terrus, Aspergillus fumigatus and Aspergillus niger, Penicillium glabrum and Cladosporium cladosporioides. A primary selection of fungi was accomplished in plate, considering Phe tolerance of every strain in two different media: potato dextrose agar (PDA) and mineral medium (MM). The radial extension rate (r r) in PDA exhibited significant differences (p < 0.05) at 200 and 400 ppm of Phe. A secondary selection of A. niger, C. cladosporoides, and P. glabrum sp. was achieved based on their tolerance to 200, 400, 600 and 800 ppm of Phe, in solid culture at a sugarcane bagasse/contaminated soil ratio of 95:5, in Toyamas, Czapeck and Wunder media. Under these conditions, a maximum (70%) Phe removal by A. niger was obtained. In addition C. cladosporioides and A. niger were able to remove high (800 ppm) Phe concentrations.
KW - Bioremediation
KW - Intergenic transcribed sequences (ITS)
KW - Phenanthrene (Phe)
KW - Polycyclic aromatic hydrocarbons (PAHs)
KW - Solid culture
UR - http://www.scopus.com/inward/record.url?scp=32944471582&partnerID=8YFLogxK
U2 - 10.1080/10934520500428351
DO - 10.1080/10934520500428351
M3 - Artículo
C2 - 16484077
SN - 1093-4529
VL - 41
SP - 475
EP - 486
JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
IS - 3
ER -