TY - JOUR
T1 - Seeds of Phaseolus vulgaris bean carry Rhizobium etli
AU - Pérez-Ramírez, Néstor O.
AU - Rogel, Marco A.
AU - Wang, Entao
AU - Castellanos, Javier Z.
AU - Martínez-Romero, Esperanza
N1 - Funding Information:
Julio Martı́nez Romero is acknowledged for technical support, Yolanda Mora for suggesting the antibiotic experiments, Michael Dunn for reviewing the manuscript; and DGAPA Grant IN202097 for partial financial support. N.O.P.R. and M.A.R. made equal contributions to the work. N.O.P.R. was a recipient of a master studentship from the IPN.
PY - 1998/8
Y1 - 1998/8
N2 - The presence of soil bacteria on seeds could provide an explanation for bacterial geographical spread. We report that Phaseolus vulgaris bean seeds naturally carry rhizobia on their testa. One hundred eighteen Rhizobium isolates from L-3-1-1-1 seeds were characterized by multilocus enzyme electrophoresis and compared to Rhizobium species which form nitrogen fixing nodules on the roots of bean plants, As a result of this and other analyses, seed isolates were classified as R. etli. Seed borne rhizobia are probably moisture limited and are acquired from contaminating soil during the harvesting process as seeds from non-opened pods do not carry them, Rhizobia on seeds are in a presumably desiccated or dormant state as they require a rehydration process during which they are antibiotic sensitive. With an improved procedure to detect rhizobia on seeds, we found that 5 out of 13 Phaseolus vulgaris bean cultivars tested harbor rhizobia on the seeds.
AB - The presence of soil bacteria on seeds could provide an explanation for bacterial geographical spread. We report that Phaseolus vulgaris bean seeds naturally carry rhizobia on their testa. One hundred eighteen Rhizobium isolates from L-3-1-1-1 seeds were characterized by multilocus enzyme electrophoresis and compared to Rhizobium species which form nitrogen fixing nodules on the roots of bean plants, As a result of this and other analyses, seed isolates were classified as R. etli. Seed borne rhizobia are probably moisture limited and are acquired from contaminating soil during the harvesting process as seeds from non-opened pods do not carry them, Rhizobia on seeds are in a presumably desiccated or dormant state as they require a rehydration process during which they are antibiotic sensitive. With an improved procedure to detect rhizobia on seeds, we found that 5 out of 13 Phaseolus vulgaris bean cultivars tested harbor rhizobia on the seeds.
KW - Bacterial spread
KW - Nitrogen fixation
KW - Phaseolus vulgaris
KW - Rhizobium
KW - Seeds
KW - Soil bacterium
UR - http://www.scopus.com/inward/record.url?scp=0345019800&partnerID=8YFLogxK
U2 - 10.1016/S0168-6496(98)00043-9
DO - 10.1016/S0168-6496(98)00043-9
M3 - Artículo
SN - 0168-6496
VL - 26
SP - 289
EP - 296
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 4
ER -