TY - JOUR
T1 - Secretion antigens of Micobacterium tuberculosis
T2 - A comparison between a reference strain and seven wild isolates
AU - Rojas-Espinosa, Oscar
AU - Rangel-Moreno, Javier
AU - Amador-Jiménez, Angélica
AU - Parra-Maldonado, Ruth
AU - Arce-Paredes, Patricia
AU - Torres-López, Javier
N1 - Funding Information:
This work was partially supported by the Dirección de Estudios Profesionales y de Investigación (DEPI) del IPN (Project nos. DEPI 970488 and DEPI 980681). AAJ and JRM are fellows of Consejo Nacional de Ciencia y Tecnologı́a (CONACYT), PAP is a fellow of COFAA (IPN) and ORE is a fellow of COFAA (IPN) and the Sistema Nacional de Investigadores (SNI), Mexico.
PY - 1999/5
Y1 - 1999/5
N2 - Background. This study was carried out with the aim of detecting possible differences between proteins secreted by fresh wild isolates of Mycobacterium tuberculosis and from a reference strain of this microorganism, H37Rv TMCC 102. Materials and Methods. This reference strain of M. tuberculosis has been in our laboratory for over 10 years, where it has been maintained by serial subcultures in PBY and Lowenstein-Jensen media. Patterns of protein secretion and recognition by sera derived from both tuberculosis patients and normal individuals were analyzed by electrophoresis and Western blotting. Results. No major qualitative differences were observed among the several strains studied with respect to protein patterns or recognition of these proteins by test sera. Normal sera were found to react with almost all antigens recognized by tuberculosis sera, but with less intensity. However, a small protein of 14.5 kDa, secreted by both the wild and reference strains of M. tuberculosis, was recognized by 32 of the 40 tuberculous patient sera tested (80%), and was not recognized by any of the 40 serum samples derived from healthy individuals. Conclusions. This small protein seems to be a potentially important antigen for the serological diagnosis of tuberculosis and/or for use in the follow-up of patients who received treatment.
AB - Background. This study was carried out with the aim of detecting possible differences between proteins secreted by fresh wild isolates of Mycobacterium tuberculosis and from a reference strain of this microorganism, H37Rv TMCC 102. Materials and Methods. This reference strain of M. tuberculosis has been in our laboratory for over 10 years, where it has been maintained by serial subcultures in PBY and Lowenstein-Jensen media. Patterns of protein secretion and recognition by sera derived from both tuberculosis patients and normal individuals were analyzed by electrophoresis and Western blotting. Results. No major qualitative differences were observed among the several strains studied with respect to protein patterns or recognition of these proteins by test sera. Normal sera were found to react with almost all antigens recognized by tuberculosis sera, but with less intensity. However, a small protein of 14.5 kDa, secreted by both the wild and reference strains of M. tuberculosis, was recognized by 32 of the 40 tuberculous patient sera tested (80%), and was not recognized by any of the 40 serum samples derived from healthy individuals. Conclusions. This small protein seems to be a potentially important antigen for the serological diagnosis of tuberculosis and/or for use in the follow-up of patients who received treatment.
KW - 14.5 kDa antigen
KW - Diagnosis of tuberculosis
KW - Mycobacteria tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=0032586967&partnerID=8YFLogxK
U2 - 10.1016/S0188-0128(99)00020-2
DO - 10.1016/S0188-0128(99)00020-2
M3 - Artículo
C2 - 10427866
AN - SCOPUS:0032586967
SN - 0188-0128
VL - 30
SP - 171
EP - 178
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 3
ER -