Scanning photoluminescent spectroscopy of bioconjugated quantum dots

G. Chornokur; S. Ostapenko; E. Oleynik; C. Phelan; N. Korsunska; T. Kryshtab; J. Zhang; A. Wolcott; T. Sellers

doi:10.1016/j.spmi.2008.11.029

Scanning photoluminescent spectroscopy of bioconjugated quantum dots

G. Chornokur, S. Ostapenko, E. Oleynik, C. Phelan, N. Korsunska, T. Kryshtab, J. Zhang, A. Wolcott, T. Sellers

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We report on the application of the bio-conjugated quantum dots (QDs) for a "sandwich" enzyme-linked immunosorbent assay (ELISA) cancer testing technique. Quantum dot ELISA detection of the cancer PSA antigen at concentrations as low as 0.01 ng/ml which is ∼50 times lower than the classic "sandwich" ELISA was demonstrated. Scanning photoluminescence (PL) spectroscopy was performed on dried ELISA wells and the results compared with the same QD samples dried on a solid substrate. We confirmed a "blue" up to 37 nm PL spectral shift in a case of QDs conjugated to PSA antibodies. Increasing of the "blue" spectral shift was observed at lower PSA antigen concentrations. The results can be used to improve sensitivity of "sandwich" ELISA cancer antigen detection.

Original language	English
Pages (from-to)	240-248
Number of pages	9
Journal	Superlattices and Microstructures
Volume	45
Issue number	4-5
DOIs	https://doi.org/10.1016/j.spmi.2008.11.029
State	Published - Apr 2009
Externally published	Yes

Keywords

Antibody
Antigen
Cancer
Photoluminescence
Quantum dots

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10.1016/j.spmi.2008.11.029

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title = "Scanning photoluminescent spectroscopy of bioconjugated quantum dots",

abstract = "We report on the application of the bio-conjugated quantum dots (QDs) for a {"}sandwich{"} enzyme-linked immunosorbent assay (ELISA) cancer testing technique. Quantum dot ELISA detection of the cancer PSA antigen at concentrations as low as 0.01 ng/ml which is ∼50 times lower than the classic {"}sandwich{"} ELISA was demonstrated. Scanning photoluminescence (PL) spectroscopy was performed on dried ELISA wells and the results compared with the same QD samples dried on a solid substrate. We confirmed a {"}blue{"} up to 37 nm PL spectral shift in a case of QDs conjugated to PSA antibodies. Increasing of the {"}blue{"} spectral shift was observed at lower PSA antigen concentrations. The results can be used to improve sensitivity of {"}sandwich{"} ELISA cancer antigen detection.",

keywords = "Antibody, Antigen, Cancer, Photoluminescence, Quantum dots",

author = "G. Chornokur and S. Ostapenko and E. Oleynik and C. Phelan and N. Korsunska and T. Kryshtab and J. Zhang and A. Wolcott and T. Sellers",

note = "Funding Information: The work was supported by National Science Foundation award DMI-0523163, Department of Defense award PC050873 and by Ministry of Education and Science of Ukraine project M/207-2007.",

year = "2009",

month = apr,

doi = "10.1016/j.spmi.2008.11.029",

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volume = "45",

pages = "240--248",

journal = "Superlattices and Microstructures",

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TY - JOUR

T1 - Scanning photoluminescent spectroscopy of bioconjugated quantum dots

AU - Chornokur, G.

AU - Ostapenko, S.

AU - Oleynik, E.

AU - Phelan, C.

AU - Korsunska, N.

AU - Kryshtab, T.

AU - Zhang, J.

AU - Wolcott, A.

AU - Sellers, T.

N1 - Funding Information: The work was supported by National Science Foundation award DMI-0523163, Department of Defense award PC050873 and by Ministry of Education and Science of Ukraine project M/207-2007.

PY - 2009/4

Y1 - 2009/4

N2 - We report on the application of the bio-conjugated quantum dots (QDs) for a "sandwich" enzyme-linked immunosorbent assay (ELISA) cancer testing technique. Quantum dot ELISA detection of the cancer PSA antigen at concentrations as low as 0.01 ng/ml which is ∼50 times lower than the classic "sandwich" ELISA was demonstrated. Scanning photoluminescence (PL) spectroscopy was performed on dried ELISA wells and the results compared with the same QD samples dried on a solid substrate. We confirmed a "blue" up to 37 nm PL spectral shift in a case of QDs conjugated to PSA antibodies. Increasing of the "blue" spectral shift was observed at lower PSA antigen concentrations. The results can be used to improve sensitivity of "sandwich" ELISA cancer antigen detection.

AB - We report on the application of the bio-conjugated quantum dots (QDs) for a "sandwich" enzyme-linked immunosorbent assay (ELISA) cancer testing technique. Quantum dot ELISA detection of the cancer PSA antigen at concentrations as low as 0.01 ng/ml which is ∼50 times lower than the classic "sandwich" ELISA was demonstrated. Scanning photoluminescence (PL) spectroscopy was performed on dried ELISA wells and the results compared with the same QD samples dried on a solid substrate. We confirmed a "blue" up to 37 nm PL spectral shift in a case of QDs conjugated to PSA antibodies. Increasing of the "blue" spectral shift was observed at lower PSA antigen concentrations. The results can be used to improve sensitivity of "sandwich" ELISA cancer antigen detection.

KW - Antibody

KW - Antigen

KW - Cancer

KW - Photoluminescence

KW - Quantum dots

UR - http://www.scopus.com/inward/record.url?scp=63149110675&partnerID=8YFLogxK

U2 - 10.1016/j.spmi.2008.11.029

DO - 10.1016/j.spmi.2008.11.029

M3 - Artículo

SN - 0749-6036

VL - 45

SP - 240

EP - 248

JO - Superlattices and Microstructures

JF - Superlattices and Microstructures

IS - 4-5

ER -

Scanning photoluminescent spectroscopy of bioconjugated quantum dots

Abstract

Keywords

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