TY - JOUR
T1 - Safety Assessment by in Vitro Digestibility and Allergenicity of Genetically Modified Maize with an Amaranth 11S Globulin
AU - Sinagawa-García, Sugey R.
AU - Rascón-Cruz, Quintín
AU - Valdez-Ortiz, Angel
AU - Medina-Godoy, Sergio
AU - Escobar-Gutiérrez, Alejandro
AU - Paredes-López, Octavio
PY - 2004/5/5
Y1 - 2004/5/5
N2 - Prospective testing for allergenicity of proteins obtained from sources with no prior history of causing allergy has been difficult to perform. Thus, the objective of this work was to assess the food safety of genetically modified maize with an amaranth globulin protein termed amarantin. Transgenic maize lines evaluated showed, in relation to nontransgenic, 4-35% more protein and 0-44% higher contents of specific essential amino acids. Individual sequence analysis with known amino acid sequences, reported as allergens, showed that none of these IgE elicitors were identified in amarantin. Amarantin was digested within the first 15 min by Simulated Gastric Fluid treatment as observed by Western blot. Expressed amarantin did not induce important levels of specific IgE antibodies in BALB/c mice, as analyzed by ELISA. We conclude that the transgenic maize with amarantin is not an important allergenicity inducer, just as nontransgenic maize.
AB - Prospective testing for allergenicity of proteins obtained from sources with no prior history of causing allergy has been difficult to perform. Thus, the objective of this work was to assess the food safety of genetically modified maize with an amaranth globulin protein termed amarantin. Transgenic maize lines evaluated showed, in relation to nontransgenic, 4-35% more protein and 0-44% higher contents of specific essential amino acids. Individual sequence analysis with known amino acid sequences, reported as allergens, showed that none of these IgE elicitors were identified in amarantin. Amarantin was digested within the first 15 min by Simulated Gastric Fluid treatment as observed by Western blot. Expressed amarantin did not induce important levels of specific IgE antibodies in BALB/c mice, as analyzed by ELISA. We conclude that the transgenic maize with amarantin is not an important allergenicity inducer, just as nontransgenic maize.
KW - Allergenicity
KW - Amaranthus
KW - Amarantin
KW - Animal model
KW - Food safety
KW - In vitro digestibility
KW - Tranagenic maize
UR - http://www.scopus.com/inward/record.url?scp=2342509708&partnerID=8YFLogxK
U2 - 10.1021/jf035487k
DO - 10.1021/jf035487k
M3 - Artículo
C2 - 15113180
SN - 0021-8561
VL - 52
SP - 2709
EP - 2714
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 9
ER -