TY - JOUR
T1 - Restoring symmetry in two-dimensional solid-state NMR correlation spectra
AU - Herbert-Pucheta, Jose Enrique
AU - Pelupessy, Philippe
AU - Bodenhausen, Geoffrey
AU - Tekely, Piotr
N1 - Funding Information:
Financial support from the Agence Nationale de la Recherche (Project ‘FastSpinProts’ ANR-09-BLAN-0111-01) and from the CNRS is gratefully acknowledged.
PY - 2012/6/29
Y1 - 2012/6/29
N2 - The intrinsic asymmetry of 2D solid-state homonuclear NMR correlation spectra that arises from a non-uniform preparation of the magnetization can be removed by equilibrating the magnetization in the initial stage of the experiments through dipolar recoupling induced by PARIS or PARISxy schemes. Both methods ensure magnetization exchange with modest radio-frequency amplitudes and permit one to create an initial state that restores the symmetry about the main diagonal of 2D spectra of uniformly labeled biomolecules. This improves the determination of structural and kinetic information. Both recoupling schemes may also be used to record nearly quantitative peak amplitudes in 1D cross-polarization magic-angle-spinning spectra.
AB - The intrinsic asymmetry of 2D solid-state homonuclear NMR correlation spectra that arises from a non-uniform preparation of the magnetization can be removed by equilibrating the magnetization in the initial stage of the experiments through dipolar recoupling induced by PARIS or PARISxy schemes. Both methods ensure magnetization exchange with modest radio-frequency amplitudes and permit one to create an initial state that restores the symmetry about the main diagonal of 2D spectra of uniformly labeled biomolecules. This improves the determination of structural and kinetic information. Both recoupling schemes may also be used to record nearly quantitative peak amplitudes in 1D cross-polarization magic-angle-spinning spectra.
UR - http://www.scopus.com/inward/record.url?scp=84862659967&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2012.05.030
DO - 10.1016/j.cplett.2012.05.030
M3 - Artículo
AN - SCOPUS:84862659967
SN - 0009-2614
VL - 539-540
SP - 245
EP - 251
JO - Chemical Physics Letters
JF - Chemical Physics Letters
ER -