Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate - Implications for the production of anti-cancer alkaloids

Ignacio E. Maldonado-Mendoza; Ronald J. Burnett; Melina Lòpez-Meyer; Craig L. Nessler

doi:10.1007/BF00033896

Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate - Implications for the production of anti-cancer alkaloids

Ignacio E. Maldonado-Mendoza, Ronald J. Burnett, Melina Lòpez-Meyer, Craig L. Nessler

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

HMGR (3-hydroxy-3-methylglutaryl-coenzyme A reductase; E.C.1.1.1.34) supplies mevalonate for the synthesis of many plant primary and secondary metabolites, including the terpenoid component of indole alkaloids. Suspension cultures of Camptotheca acuminata and Catharanthus roseus, two species valued for their anticancer indole alkaloids, were treated with the elicitation signal transducer methyl jasmonate (MeJA). RNA gel blot analysis from MeJA treated cultures showed a transient suppression of HMGR mRNA, followed by an induction in HMGR message. Leaf disks from transgenic tobacco plants containing a chimeric hmgl::GUS construct were also treated with MeJA and showed a dose dependent suppression of wound-inducible GUS activity. The suppression of the wound response by MeJA was limited to the first 4 h post-wounding, after which time MeJA application had no effect. The results are discussed in relation to the differential regulation of HMGR isogenes in higher plants.

Original language	English
Pages (from-to)	351-356
Number of pages	6
Journal	Plant Cell, Tissue and Organ Culture
Volume	38
Issue number	2-3
DOIs	https://doi.org/10.1007/BF00033896
State	Published - Sep 1994
Externally published	Yes

Keywords

Camptotheca acuminata
Catharanthus roseus
HMGR
methyl jasmonate
terpenoid indole alkaloids

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10.1007/BF00033896

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title = "Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate - Implications for the production of anti-cancer alkaloids",

abstract = "HMGR (3-hydroxy-3-methylglutaryl-coenzyme A reductase; E.C.1.1.1.34) supplies mevalonate for the synthesis of many plant primary and secondary metabolites, including the terpenoid component of indole alkaloids. Suspension cultures of Camptotheca acuminata and Catharanthus roseus, two species valued for their anticancer indole alkaloids, were treated with the elicitation signal transducer methyl jasmonate (MeJA). RNA gel blot analysis from MeJA treated cultures showed a transient suppression of HMGR mRNA, followed by an induction in HMGR message. Leaf disks from transgenic tobacco plants containing a chimeric hmgl::GUS construct were also treated with MeJA and showed a dose dependent suppression of wound-inducible GUS activity. The suppression of the wound response by MeJA was limited to the first 4 h post-wounding, after which time MeJA application had no effect. The results are discussed in relation to the differential regulation of HMGR isogenes in higher plants.",

keywords = "Camptotheca acuminata, Catharanthus roseus, HMGR, methyl jasmonate, terpenoid indole alkaloids",

author = "Maldonado-Mendoza, {Ignacio E.} and Burnett, {Ronald J.} and Melina L{\`o}pez-Meyer and Nessler, {Craig L.}",

year = "1994",

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doi = "10.1007/BF00033896",

language = "Ingl{\'e}s",

volume = "38",

pages = "351--356",

journal = "Plant Cell, Tissue and Organ Culture",

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Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate - Implications for the production of anti-cancer alkaloids. / Maldonado-Mendoza, Ignacio E.; Burnett, Ronald J.; Lòpez-Meyer, Melina et al.
In: Plant Cell, Tissue and Organ Culture, Vol. 38, No. 2-3, 09.1994, p. 351-356.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate - Implications for the production of anti-cancer alkaloids

AU - Maldonado-Mendoza, Ignacio E.

AU - Burnett, Ronald J.

AU - Lòpez-Meyer, Melina

AU - Nessler, Craig L.

PY - 1994/9

Y1 - 1994/9

N2 - HMGR (3-hydroxy-3-methylglutaryl-coenzyme A reductase; E.C.1.1.1.34) supplies mevalonate for the synthesis of many plant primary and secondary metabolites, including the terpenoid component of indole alkaloids. Suspension cultures of Camptotheca acuminata and Catharanthus roseus, two species valued for their anticancer indole alkaloids, were treated with the elicitation signal transducer methyl jasmonate (MeJA). RNA gel blot analysis from MeJA treated cultures showed a transient suppression of HMGR mRNA, followed by an induction in HMGR message. Leaf disks from transgenic tobacco plants containing a chimeric hmgl::GUS construct were also treated with MeJA and showed a dose dependent suppression of wound-inducible GUS activity. The suppression of the wound response by MeJA was limited to the first 4 h post-wounding, after which time MeJA application had no effect. The results are discussed in relation to the differential regulation of HMGR isogenes in higher plants.

AB - HMGR (3-hydroxy-3-methylglutaryl-coenzyme A reductase; E.C.1.1.1.34) supplies mevalonate for the synthesis of many plant primary and secondary metabolites, including the terpenoid component of indole alkaloids. Suspension cultures of Camptotheca acuminata and Catharanthus roseus, two species valued for their anticancer indole alkaloids, were treated with the elicitation signal transducer methyl jasmonate (MeJA). RNA gel blot analysis from MeJA treated cultures showed a transient suppression of HMGR mRNA, followed by an induction in HMGR message. Leaf disks from transgenic tobacco plants containing a chimeric hmgl::GUS construct were also treated with MeJA and showed a dose dependent suppression of wound-inducible GUS activity. The suppression of the wound response by MeJA was limited to the first 4 h post-wounding, after which time MeJA application had no effect. The results are discussed in relation to the differential regulation of HMGR isogenes in higher plants.

KW - Camptotheca acuminata

KW - Catharanthus roseus

KW - HMGR

KW - methyl jasmonate

KW - terpenoid indole alkaloids

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DO - 10.1007/BF00033896

M3 - Artículo

SN - 0167-6857

VL - 38

SP - 351

EP - 356

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 2-3

ER -

Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate - Implications for the production of anti-cancer alkaloids

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Keywords

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