TY - JOUR
T1 - Recent Insights in Pre-mRNA 3 ́-End processing signals and proteins in the protozoan parasite Entamoeba histolytica
AU - López-Camarillo, César
AU - de la Cruz, Olga N.Hernández
AU - Vivas, Jessica García
AU - Retana, Jorge Fernández
AU - Valdez, Marisol Pezet
AU - Rosas, Itzel Lopez
AU - Alvarez-Sánchez, Elizbeth
AU - Marchat, Laurence A.
PY - 2010
Y1 - 2010
N2 - The messenger RNA precursors (pre-mRNA) 3 ́-end processing occurs in a two-step co-transcriptional coupled reaction, denoted as cleavage and polyadenylation. Both processes depend on trans-acting factors interacting in a coordinated manner with cis-sequence motifs located at the 3 ́ untranslated region of transcripts. In this paper, we reviewed mechanisms involved in pre-mRNA processing in eukaryotic organisms, including our own findings about sequences and proteins potentially involved in mRNA 3 ́-end formation in the protozoan parasite Entamoeba histolytica. Interestingly, protein sequence comparisons among E. histolytica, yeast, and human pre-mRNA processing machineries showed that amoeba pre-mRNA 3 ́-end processing machinery appears to be in an intermediate evolutionary position between mammals and yeast. In addition, the presence of non canonical poly(A) polymerases family recently identified in E. histolytica, adds more complexity to the mRNA 3 ́-end formation process in this ancient eukaryote.
AB - The messenger RNA precursors (pre-mRNA) 3 ́-end processing occurs in a two-step co-transcriptional coupled reaction, denoted as cleavage and polyadenylation. Both processes depend on trans-acting factors interacting in a coordinated manner with cis-sequence motifs located at the 3 ́ untranslated region of transcripts. In this paper, we reviewed mechanisms involved in pre-mRNA processing in eukaryotic organisms, including our own findings about sequences and proteins potentially involved in mRNA 3 ́-end formation in the protozoan parasite Entamoeba histolytica. Interestingly, protein sequence comparisons among E. histolytica, yeast, and human pre-mRNA processing machineries showed that amoeba pre-mRNA 3 ́-end processing machinery appears to be in an intermediate evolutionary position between mammals and yeast. In addition, the presence of non canonical poly(A) polymerases family recently identified in E. histolytica, adds more complexity to the mRNA 3 ́-end formation process in this ancient eukaryote.
KW - Amoebiasis
KW - Entamoeba histolytica
KW - Polyadenylation
KW - Posttranscriptional regulation
KW - Pre-mRNA processing
UR - http://www.scopus.com/inward/record.url?scp=77958518154&partnerID=8YFLogxK
U2 - 10.2174/187152610791591575
DO - 10.2174/187152610791591575
M3 - Artículo de revisión
SN - 1871-5265
VL - 10
SP - 258
EP - 265
JO - Infectious Disorders - Drug Targets
JF - Infectious Disorders - Drug Targets
IS - 4
ER -