TY - JOUR
T1 - Realistic concentrations of Bisphenol-A trigger a neurotoxic response in the brain of zebrafish
T2 - Oxidative stress, behavioral impairment, acetylcholinesterase inhibition, and gene expression disruption
AU - Heredia-García, Gerardo
AU - Elizalde-Velázquez, Gustavo Axel
AU - Gómez-Oliván, Leobardo Manuel
AU - Islas-Flores, Hariz
AU - García-Medina, Sandra
AU - Galar-Martínez, Marcela
AU - Dublán-García, Octavio
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/7
Y1 - 2023/7
N2 - Bisphenol A (BPA) is a micro-pollutant found in various environmental matrices at concentrations as low as ng/L. Recent studies have shown that this compound can cause oxidative damage and neurotoxic effects in aquatic organisms. However, there is a lack of research investigating the effects of BPA at environmentally relevant concentrations. Therefore, this study aimed to assess the neurotoxic effects of acute BPA exposure (96 h) at environmentally relevant concentrations (220, 1180, and 1500 ng/L) in adult zebrafish (Danio rerio). The Novel Tank trial was used to evaluate fish swimming behavior, and our results indicate that exposure to 1500 ng/L of BPA reduced the total distance traveled and increased freezing time. Furthermore, the evaluation of biomarkers in the zebrafish brain revealed that BPA exposure led to the production of reactive oxygen species and increased acetylcholinesterase activity. Gene expression analysis also indicated the overexpression of mbp, α1-tubulin, and manf in the zebrafish brain. Based on our findings, we concluded that environmentally relevant concentrations of BPA can cause anxiety-like behavior and neurotoxic effects in adult zebrafish.
AB - Bisphenol A (BPA) is a micro-pollutant found in various environmental matrices at concentrations as low as ng/L. Recent studies have shown that this compound can cause oxidative damage and neurotoxic effects in aquatic organisms. However, there is a lack of research investigating the effects of BPA at environmentally relevant concentrations. Therefore, this study aimed to assess the neurotoxic effects of acute BPA exposure (96 h) at environmentally relevant concentrations (220, 1180, and 1500 ng/L) in adult zebrafish (Danio rerio). The Novel Tank trial was used to evaluate fish swimming behavior, and our results indicate that exposure to 1500 ng/L of BPA reduced the total distance traveled and increased freezing time. Furthermore, the evaluation of biomarkers in the zebrafish brain revealed that BPA exposure led to the production of reactive oxygen species and increased acetylcholinesterase activity. Gene expression analysis also indicated the overexpression of mbp, α1-tubulin, and manf in the zebrafish brain. Based on our findings, we concluded that environmentally relevant concentrations of BPA can cause anxiety-like behavior and neurotoxic effects in adult zebrafish.
KW - BPA
KW - Behavioral alterations
KW - Fish
KW - Neurotoxicity
KW - Oxidative damage
UR - http://www.scopus.com/inward/record.url?scp=85152894339&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2023.138729
DO - 10.1016/j.chemosphere.2023.138729
M3 - Artículo
C2 - 37080469
AN - SCOPUS:85152894339
SN - 0045-6535
VL - 330
JO - Chemosphere
JF - Chemosphere
M1 - 138729
ER -