Abstract
Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l-1, whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l-1. The particle median diameter decreased from 2 to 0.5 mm as Tween concentration was increased and was inversely correlated to the biomass dry weight. Adding pre-determined aliquots of Tween 40 to the culture medium can be used to define the morphological characteristics of Trichoderma harzianum in submerged shake-flask culture.
Original language | English |
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Pages (from-to) | 41-44 |
Number of pages | 4 |
Journal | Biotechnology Letters |
Volume | 26 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2004 |
Keywords
- Fungi
- Morphology
- Shake-flasks
- Trichoderma
- Tween