TY - JOUR
T1 - Quantitative and qualitative peritoneal immune profiles, T-cell apoptosis and oxidative stress-associated characteristics in women with minimal and mild endometriosis
AU - Mier-Cabrera, Jennifer
AU - Jiménez-Zamudio, Luis
AU - García-Latorre, Ethel
AU - Cruz-Orozco, Oliver
AU - Hernández-Guerrero, César
PY - 2011/1
Y1 - 2011/1
N2 - Objectives To assess immunological variables, T-cell apoptosis and oxidative stress markers in the peripheral blood and peritoneal fluid of women with (WEN) and without (WWE) endometriosis. Design: Observational and transverse case-control study. Setting: National Institute of Perinatology, Mexico City, Mexico. Population: and sample Peripheral blood and peritoneal fluid obtained from 30 WWE and 32 WEN. Methods: Blood was drawn before surgery and peritoneal fluid was collected during surgery but before any surgical procedure had been carried out. Flow cytometry, spectrophotometry, high-performance liquid chromatography and multiplex immunoassay analyses were performed. Main outcome measures: Peripheral and peritoneal lymphocyte subpopulations (CD3+, CD4+ CD3+, CD8+ CD3+, CD16 + CD56+, human leucocyte antigen-DR+ CD3 + and CD19+), intracellular CD4+ CD3 + and CD8+ CD3+ cytokine synthesis (interleukin-2 [IL-2] and interferon-γ [IFN-γ]), CD3+ apoptosis, malondialdehyde and ascorbate concentrations and peritoneal cytokine concentrations. Results: No differences were found in peripheral and peritoneal lymphocyte subsets between the groups. Peritoneal T lymphocytes from WEN produced less IL-2 and IFN-γ than those from WWE. Peritoneal malondialdehyde concentrations were higher and ascorbate concentrations were lower in WEN than in WWE. Higher peritoneal concentrations of pro-inflammatory cytokines (IL-1β, tumour necrosis factor-α and IL-6) and chemokines (IL-10, IL-8, eotaxin, vascular endothelial growth factor, monocyte chemotactic protein-1 and regulated upon activation, normal T-cell expressed, and secreted) and lower concentrations of IFN-γ, IL-1 receptor antagonist and IL-15 were found in WEN. No statistical differences were found in IL-2, IL-4, IL-12 and IL-13 concentrations. Conclusion The alterations observed in WEN were associated with a diminished peritoneal T helper type 1 immune response. Pro-inflammatory, chemotactic, angiogenic and oxidative stress markers were altered in the peritoneal milieu of WEN. These changes appeared to contribute to the peritoneal immune alterations found.
AB - Objectives To assess immunological variables, T-cell apoptosis and oxidative stress markers in the peripheral blood and peritoneal fluid of women with (WEN) and without (WWE) endometriosis. Design: Observational and transverse case-control study. Setting: National Institute of Perinatology, Mexico City, Mexico. Population: and sample Peripheral blood and peritoneal fluid obtained from 30 WWE and 32 WEN. Methods: Blood was drawn before surgery and peritoneal fluid was collected during surgery but before any surgical procedure had been carried out. Flow cytometry, spectrophotometry, high-performance liquid chromatography and multiplex immunoassay analyses were performed. Main outcome measures: Peripheral and peritoneal lymphocyte subpopulations (CD3+, CD4+ CD3+, CD8+ CD3+, CD16 + CD56+, human leucocyte antigen-DR+ CD3 + and CD19+), intracellular CD4+ CD3 + and CD8+ CD3+ cytokine synthesis (interleukin-2 [IL-2] and interferon-γ [IFN-γ]), CD3+ apoptosis, malondialdehyde and ascorbate concentrations and peritoneal cytokine concentrations. Results: No differences were found in peripheral and peritoneal lymphocyte subsets between the groups. Peritoneal T lymphocytes from WEN produced less IL-2 and IFN-γ than those from WWE. Peritoneal malondialdehyde concentrations were higher and ascorbate concentrations were lower in WEN than in WWE. Higher peritoneal concentrations of pro-inflammatory cytokines (IL-1β, tumour necrosis factor-α and IL-6) and chemokines (IL-10, IL-8, eotaxin, vascular endothelial growth factor, monocyte chemotactic protein-1 and regulated upon activation, normal T-cell expressed, and secreted) and lower concentrations of IFN-γ, IL-1 receptor antagonist and IL-15 were found in WEN. No statistical differences were found in IL-2, IL-4, IL-12 and IL-13 concentrations. Conclusion The alterations observed in WEN were associated with a diminished peritoneal T helper type 1 immune response. Pro-inflammatory, chemotactic, angiogenic and oxidative stress markers were altered in the peritoneal milieu of WEN. These changes appeared to contribute to the peritoneal immune alterations found.
KW - Apoptosis
KW - endometriosis
KW - intracellular cytokines
KW - lymphocyte subpopulations
KW - multiplex immunoassay
UR - http://www.scopus.com/inward/record.url?scp=78650268992&partnerID=8YFLogxK
U2 - 10.1111/j.1471-0528.2010.02777.x
DO - 10.1111/j.1471-0528.2010.02777.x
M3 - Artículo
C2 - 21083865
SN - 1470-0328
VL - 118
SP - 6
EP - 16
JO - BJOG: An International Journal of Obstetrics and Gynaecology
JF - BJOG: An International Journal of Obstetrics and Gynaecology
IS - 1
ER -