Quantitative and qualitative peritoneal immune profiles, T-cell apoptosis and oxidative stress-associated characteristics in women with minimal and mild endometriosis

Jennifer Mier-Cabrera, Luis Jiménez-Zamudio, Ethel García-Latorre, Oliver Cruz-Orozco, César Hernández-Guerrero

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Abstract

Objectives To assess immunological variables, T-cell apoptosis and oxidative stress markers in the peripheral blood and peritoneal fluid of women with (WEN) and without (WWE) endometriosis. Design: Observational and transverse case-control study. Setting: National Institute of Perinatology, Mexico City, Mexico. Population: and sample Peripheral blood and peritoneal fluid obtained from 30 WWE and 32 WEN. Methods: Blood was drawn before surgery and peritoneal fluid was collected during surgery but before any surgical procedure had been carried out. Flow cytometry, spectrophotometry, high-performance liquid chromatography and multiplex immunoassay analyses were performed. Main outcome measures: Peripheral and peritoneal lymphocyte subpopulations (CD3 + , CD4 + CD3 + , CD8 + CD3 + , CD16 + CD56 + , human leucocyte antigen-DR + CD3 + and CD19 + ), intracellular CD4 + CD3 + and CD8 + CD3 + cytokine synthesis (interleukin-2 [IL-2] and interferon-γ [IFN-γ] ), CD3 + apoptosis, malondialdehyde and ascorbate concentrations and peritoneal cytokine concentrations. Results: No differences were found in peripheral and peritoneal lymphocyte subsets between the groups. Peritoneal T lymphocytes from WEN produced less IL-2 and IFN-γ than those from WWE. Peritoneal malondialdehyde concentrations were higher and ascorbate concentrations were lower in WEN than in WWE. Higher peritoneal concentrations of pro-inflammatory cytokines (IL-1β, tumour necrosis factor-α and IL-6) and chemokines (IL-10, IL-8, eotaxin, vascular endothelial growth factor, monocyte chemotactic protein-1 and regulated upon activation, normal T-cell expressed, and secreted) and lower concentrations of IFN-γ, IL-1 receptor antagonist and IL-15 were found in WEN. No statistical differences were found in IL-2, IL-4, IL-12 and IL-13 concentrations. Conclusion The alterations observed in WEN were associated with a diminished peritoneal T helper type 1 immune response. Pro-inflammatory, chemotactic, angiogenic and oxidative stress markers were altered in the peritoneal milieu of WEN. These changes appeared to contribute to the peritoneal immune alterations found. © RCOG 2010 BJOG An International Journal of Obstetrics and Gynaecology.
Original languageAmerican English
Pages (from-to)6-16
Number of pages11
JournalBJOG: An International Journal of Obstetrics and Gynaecology
DOIs
StatePublished - 1 Jan 2011

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Endometriosis
Oxidative Stress
Apoptosis
T-Lymphocytes
Ascitic Fluid
Interferons
Interleukin-2
Lymphocyte Subsets
Cytokines
Mexico
Malondialdehyde
Perinatology
Interleukin-15
Interleukin-1 Receptors
Interleukin-13
Chemokine CCL2
Spectrophotometry
Interleukin-12
HLA Antigens
Gynecology

Cite this

@article{990d7a7232f74f1688cb6d90415b2aa8,
title = "Quantitative and qualitative peritoneal immune profiles, T-cell apoptosis and oxidative stress-associated characteristics in women with minimal and mild endometriosis",
abstract = "Objectives To assess immunological variables, T-cell apoptosis and oxidative stress markers in the peripheral blood and peritoneal fluid of women with (WEN) and without (WWE) endometriosis. Design: Observational and transverse case-control study. Setting: National Institute of Perinatology, Mexico City, Mexico. Population: and sample Peripheral blood and peritoneal fluid obtained from 30 WWE and 32 WEN. Methods: Blood was drawn before surgery and peritoneal fluid was collected during surgery but before any surgical procedure had been carried out. Flow cytometry, spectrophotometry, high-performance liquid chromatography and multiplex immunoassay analyses were performed. Main outcome measures: Peripheral and peritoneal lymphocyte subpopulations (CD3 + , CD4 + CD3 + , CD8 + CD3 + , CD16 + CD56 + , human leucocyte antigen-DR + CD3 + and CD19 + ), intracellular CD4 + CD3 + and CD8 + CD3 + cytokine synthesis (interleukin-2 [IL-2] and interferon-γ [IFN-γ] ), CD3 + apoptosis, malondialdehyde and ascorbate concentrations and peritoneal cytokine concentrations. Results: No differences were found in peripheral and peritoneal lymphocyte subsets between the groups. Peritoneal T lymphocytes from WEN produced less IL-2 and IFN-γ than those from WWE. Peritoneal malondialdehyde concentrations were higher and ascorbate concentrations were lower in WEN than in WWE. Higher peritoneal concentrations of pro-inflammatory cytokines (IL-1β, tumour necrosis factor-α and IL-6) and chemokines (IL-10, IL-8, eotaxin, vascular endothelial growth factor, monocyte chemotactic protein-1 and regulated upon activation, normal T-cell expressed, and secreted) and lower concentrations of IFN-γ, IL-1 receptor antagonist and IL-15 were found in WEN. No statistical differences were found in IL-2, IL-4, IL-12 and IL-13 concentrations. Conclusion The alterations observed in WEN were associated with a diminished peritoneal T helper type 1 immune response. Pro-inflammatory, chemotactic, angiogenic and oxidative stress markers were altered in the peritoneal milieu of WEN. These changes appeared to contribute to the peritoneal immune alterations found. {\circledC} RCOG 2010 BJOG An International Journal of Obstetrics and Gynaecology.",
author = "Jennifer Mier-Cabrera and Luis Jim{\'e}nez-Zamudio and Ethel Garc{\'i}a-Latorre and Oliver Cruz-Orozco and C{\'e}sar Hern{\'a}ndez-Guerrero",
year = "2011",
month = "1",
day = "1",
doi = "10.1111/j.1471-0528.2010.02777.x",
language = "American English",
pages = "6--16",
journal = "BJOG: An International Journal of Obstetrics and Gynaecology",
issn = "1470-0328",
publisher = "Wiley-Blackwell Publishing Ltd",

}

TY - JOUR

T1 - Quantitative and qualitative peritoneal immune profiles, T-cell apoptosis and oxidative stress-associated characteristics in women with minimal and mild endometriosis

AU - Mier-Cabrera, Jennifer

AU - Jiménez-Zamudio, Luis

AU - García-Latorre, Ethel

AU - Cruz-Orozco, Oliver

AU - Hernández-Guerrero, César

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Objectives To assess immunological variables, T-cell apoptosis and oxidative stress markers in the peripheral blood and peritoneal fluid of women with (WEN) and without (WWE) endometriosis. Design: Observational and transverse case-control study. Setting: National Institute of Perinatology, Mexico City, Mexico. Population: and sample Peripheral blood and peritoneal fluid obtained from 30 WWE and 32 WEN. Methods: Blood was drawn before surgery and peritoneal fluid was collected during surgery but before any surgical procedure had been carried out. Flow cytometry, spectrophotometry, high-performance liquid chromatography and multiplex immunoassay analyses were performed. Main outcome measures: Peripheral and peritoneal lymphocyte subpopulations (CD3 + , CD4 + CD3 + , CD8 + CD3 + , CD16 + CD56 + , human leucocyte antigen-DR + CD3 + and CD19 + ), intracellular CD4 + CD3 + and CD8 + CD3 + cytokine synthesis (interleukin-2 [IL-2] and interferon-γ [IFN-γ] ), CD3 + apoptosis, malondialdehyde and ascorbate concentrations and peritoneal cytokine concentrations. Results: No differences were found in peripheral and peritoneal lymphocyte subsets between the groups. Peritoneal T lymphocytes from WEN produced less IL-2 and IFN-γ than those from WWE. Peritoneal malondialdehyde concentrations were higher and ascorbate concentrations were lower in WEN than in WWE. Higher peritoneal concentrations of pro-inflammatory cytokines (IL-1β, tumour necrosis factor-α and IL-6) and chemokines (IL-10, IL-8, eotaxin, vascular endothelial growth factor, monocyte chemotactic protein-1 and regulated upon activation, normal T-cell expressed, and secreted) and lower concentrations of IFN-γ, IL-1 receptor antagonist and IL-15 were found in WEN. No statistical differences were found in IL-2, IL-4, IL-12 and IL-13 concentrations. Conclusion The alterations observed in WEN were associated with a diminished peritoneal T helper type 1 immune response. Pro-inflammatory, chemotactic, angiogenic and oxidative stress markers were altered in the peritoneal milieu of WEN. These changes appeared to contribute to the peritoneal immune alterations found. © RCOG 2010 BJOG An International Journal of Obstetrics and Gynaecology.

AB - Objectives To assess immunological variables, T-cell apoptosis and oxidative stress markers in the peripheral blood and peritoneal fluid of women with (WEN) and without (WWE) endometriosis. Design: Observational and transverse case-control study. Setting: National Institute of Perinatology, Mexico City, Mexico. Population: and sample Peripheral blood and peritoneal fluid obtained from 30 WWE and 32 WEN. Methods: Blood was drawn before surgery and peritoneal fluid was collected during surgery but before any surgical procedure had been carried out. Flow cytometry, spectrophotometry, high-performance liquid chromatography and multiplex immunoassay analyses were performed. Main outcome measures: Peripheral and peritoneal lymphocyte subpopulations (CD3 + , CD4 + CD3 + , CD8 + CD3 + , CD16 + CD56 + , human leucocyte antigen-DR + CD3 + and CD19 + ), intracellular CD4 + CD3 + and CD8 + CD3 + cytokine synthesis (interleukin-2 [IL-2] and interferon-γ [IFN-γ] ), CD3 + apoptosis, malondialdehyde and ascorbate concentrations and peritoneal cytokine concentrations. Results: No differences were found in peripheral and peritoneal lymphocyte subsets between the groups. Peritoneal T lymphocytes from WEN produced less IL-2 and IFN-γ than those from WWE. Peritoneal malondialdehyde concentrations were higher and ascorbate concentrations were lower in WEN than in WWE. Higher peritoneal concentrations of pro-inflammatory cytokines (IL-1β, tumour necrosis factor-α and IL-6) and chemokines (IL-10, IL-8, eotaxin, vascular endothelial growth factor, monocyte chemotactic protein-1 and regulated upon activation, normal T-cell expressed, and secreted) and lower concentrations of IFN-γ, IL-1 receptor antagonist and IL-15 were found in WEN. No statistical differences were found in IL-2, IL-4, IL-12 and IL-13 concentrations. Conclusion The alterations observed in WEN were associated with a diminished peritoneal T helper type 1 immune response. Pro-inflammatory, chemotactic, angiogenic and oxidative stress markers were altered in the peritoneal milieu of WEN. These changes appeared to contribute to the peritoneal immune alterations found. © RCOG 2010 BJOG An International Journal of Obstetrics and Gynaecology.

U2 - 10.1111/j.1471-0528.2010.02777.x

DO - 10.1111/j.1471-0528.2010.02777.x

M3 - Article

C2 - 21083865

SP - 6

EP - 16

JO - BJOG: An International Journal of Obstetrics and Gynaecology

JF - BJOG: An International Journal of Obstetrics and Gynaecology

SN - 1470-0328

ER -