TY - JOUR
T1 - PvLOX2 silencing in common bean roots impairs arbuscular mycorrhiza-induced resistance without affecting symbiosis establishment
AU - Mora-Romero, Guadalupe A.
AU - Gonzalez-Ortiz, Maria A.
AU - Quiroz-Figueroa, Francisco
AU - Calderon-Vazquez, Carlos L.
AU - Medina-Godoy, Sergio
AU - Maldonado-Mendoza, Ignacio
AU - Arroyo-Becerra, Analilia
AU - Perez-Torres, Anahí
AU - Alatorre-Cobos, Fulgencio
AU - Sanchez, Federico
AU - Lopez-Meyer, Melina
N1 - Publisher Copyright:
© CSIRO 2015.
PY - 2015
Y1 - 2015
N2 - The arbuscular mycorrhizal (AM) symbiosis is an intimate association between specific soil-borne fungi and the roots of most land plants. AM colonisation elicits an enhanced defence resistance against pathogens, known as mycorrhizal-induced resistance (MIR). This mechanism locally and systemically sensitises plant tissues to boost their basal defence response. Although a role for oxylipins in MIR has been proposed, it has not yet been experimentally confirmed. In this study, when the common bean (Phaseolus vulgaris L.) lipoxygenase PvLOX2 was silenced in roots of composite plants, leaves of silenced plants lost their capacity to exhibit MIR against the foliar pathogen Sclerotinia sclerotiorum, even though they were colonised normally. PvLOX6, a LOX gene family member, is involved in JA biosynthesis in the common bean. Downregulation of PvLOX2 and PvLOX6 in leaves of PvLOX2 root-silenced plants coincides with the loss of MIR, suggesting that these genes could be involved in the onset and spreading of the mycorrhiza-induced defence response.
AB - The arbuscular mycorrhizal (AM) symbiosis is an intimate association between specific soil-borne fungi and the roots of most land plants. AM colonisation elicits an enhanced defence resistance against pathogens, known as mycorrhizal-induced resistance (MIR). This mechanism locally and systemically sensitises plant tissues to boost their basal defence response. Although a role for oxylipins in MIR has been proposed, it has not yet been experimentally confirmed. In this study, when the common bean (Phaseolus vulgaris L.) lipoxygenase PvLOX2 was silenced in roots of composite plants, leaves of silenced plants lost their capacity to exhibit MIR against the foliar pathogen Sclerotinia sclerotiorum, even though they were colonised normally. PvLOX6, a LOX gene family member, is involved in JA biosynthesis in the common bean. Downregulation of PvLOX2 and PvLOX6 in leaves of PvLOX2 root-silenced plants coincides with the loss of MIR, suggesting that these genes could be involved in the onset and spreading of the mycorrhiza-induced defence response.
KW - RNA silencing
KW - fungi
KW - systemic resistance.
UR - http://www.scopus.com/inward/record.url?scp=84916917665&partnerID=8YFLogxK
U2 - 10.1071/FP14101
DO - 10.1071/FP14101
M3 - Artículo
SN - 1445-4408
VL - 42
SP - 18
EP - 30
JO - Functional Plant Biology
JF - Functional Plant Biology
IS - 1
ER -