Purification and cellular localization of the Entamoeba histolytica transcarboxylase

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Abstract

Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation. © Springer-Verlag 2012.
Original languageAmerican English
Pages (from-to)1401-1405
Number of pages1260
JournalParasitology Research
DOIs
StatePublished - 1 Sep 2012

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Methylmalonyl-CoA carboxytransferase
Entamoeba histolytica
biotin carboxylase
biotin
Acetyl-CoA Carboxylase
acetyl-CoA carboxylase
histochemistry
Biotin
Confocal Microscopy
Cytoplasm
Western blotting
Proteins
Fatty Acids
cytoplasm
proteins
Molecular Weight
Western Blotting
Genome
molecular weight
fatty acids

Cite this

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title = "Purification and cellular localization of the Entamoeba histolytica transcarboxylase",
abstract = "Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation. {\circledC} Springer-Verlag 2012.",
author = "E. Barbosa-Cabrera and A. Salas-Casas and S. Rojas-Hern{\'a}ndez and A. Jarillo-Luna and E. Abarca-Rojano and Rodr{\'i}guez, {M. A.} and R. Campos-Rodr{\'i}guez",
year = "2012",
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doi = "10.1007/s00436-012-2898-6",
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TY - JOUR

T1 - Purification and cellular localization of the Entamoeba histolytica transcarboxylase

AU - Barbosa-Cabrera, E.

AU - Salas-Casas, A.

AU - Rojas-Hernández, S.

AU - Jarillo-Luna, A.

AU - Abarca-Rojano, E.

AU - Rodríguez, M. A.

AU - Campos-Rodríguez, R.

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation. © Springer-Verlag 2012.

AB - Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation. © Springer-Verlag 2012.

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U2 - 10.1007/s00436-012-2898-6

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