Pulsed laser deposition of bovine serum albumin protein thin films

M. A. Hernandez-Perez; C. Garapon; C. Champeaux; P. Shahgaldian; A. Coleman; J. Mugnier

doi:10.1016/S0169-4332(02)01419-8

Pulsed laser deposition of bovine serum albumin protein thin films

M. A. Hernandez-Perez, C. Garapon, C. Champeaux, P. Shahgaldian, A. Coleman, J. Mugnier

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Thin films of the bovine serum albumin protein (BSA) were prepared by pulsed laser deposition (PLD). Using a KrF excimer laser beam focused on a target made of pressed powder with a fluence in the 1-2 J cm ^-2 range, films of about 1μm thickness and low roughness could be obtained on silica substrates at room temperature and under a pressure of about 10 ^-5 mbar. The Fourier transform infrared (FTIR) spectra of the films, recorded in attenuated total reflection (ATR) configuration, are nearly identical to those of the starting powders. For fluences lower than 0.6 J cm ^-2 , however, the structure of the molecule is not preserved. Atomic force microscopy (AFM) images show the protein to be deposited as particles of about 100 nm diameter without clear ordering and the films to have low roughness. Refractive index and thickness of the films were measured by m-lines spectroscopy.

Original language	English
Pages (from-to)	658-662
Number of pages	5
Journal	Applied Surface Science
Volume	208-209
Issue number	1
DOIs	https://doi.org/10.1016/S0169-4332(02)01419-8
State	Published - 15 Mar 2003
Externally published	Yes

Keywords

AFM
FTIR
Laser deposition
Protein
Thin films

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10.1016/S0169-4332(02)01419-8

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title = "Pulsed laser deposition of bovine serum albumin protein thin films",

abstract = " Thin films of the bovine serum albumin protein (BSA) were prepared by pulsed laser deposition (PLD). Using a KrF excimer laser beam focused on a target made of pressed powder with a fluence in the 1-2 J cm -2 range, films of about 1μm thickness and low roughness could be obtained on silica substrates at room temperature and under a pressure of about 10 -5 mbar. The Fourier transform infrared (FTIR) spectra of the films, recorded in attenuated total reflection (ATR) configuration, are nearly identical to those of the starting powders. For fluences lower than 0.6 J cm -2 , however, the structure of the molecule is not preserved. Atomic force microscopy (AFM) images show the protein to be deposited as particles of about 100 nm diameter without clear ordering and the films to have low roughness. Refractive index and thickness of the films were measured by m-lines spectroscopy.",

keywords = "AFM, FTIR, Laser deposition, Protein, Thin films",

author = "Hernandez-Perez, {M. A.} and C. Garapon and C. Champeaux and P. Shahgaldian and A. Coleman and J. Mugnier",

note = "Funding Information: This research was supported by CNRS (Action Coordonn{\'e}e “Optique, G{\'e}nie des proc{\'e}d{\'e}s, Mat{\'e}riaux”). Fruitful discussions with Y. Mar{\'e}chal (DRFMC/CEA-Grenoble) is gratefully acknowledged. ",

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day = "15",

doi = "10.1016/S0169-4332(02)01419-8",

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volume = "208-209",

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T1 - Pulsed laser deposition of bovine serum albumin protein thin films

AU - Hernandez-Perez, M. A.

AU - Garapon, C.

AU - Champeaux, C.

AU - Shahgaldian, P.

AU - Coleman, A.

AU - Mugnier, J.

N1 - Funding Information: This research was supported by CNRS (Action Coordonnée “Optique, Génie des procédés, Matériaux”). Fruitful discussions with Y. Maréchal (DRFMC/CEA-Grenoble) is gratefully acknowledged.

PY - 2003/3/15

Y1 - 2003/3/15

N2 - Thin films of the bovine serum albumin protein (BSA) were prepared by pulsed laser deposition (PLD). Using a KrF excimer laser beam focused on a target made of pressed powder with a fluence in the 1-2 J cm -2 range, films of about 1μm thickness and low roughness could be obtained on silica substrates at room temperature and under a pressure of about 10 -5 mbar. The Fourier transform infrared (FTIR) spectra of the films, recorded in attenuated total reflection (ATR) configuration, are nearly identical to those of the starting powders. For fluences lower than 0.6 J cm -2 , however, the structure of the molecule is not preserved. Atomic force microscopy (AFM) images show the protein to be deposited as particles of about 100 nm diameter without clear ordering and the films to have low roughness. Refractive index and thickness of the films were measured by m-lines spectroscopy.

AB - Thin films of the bovine serum albumin protein (BSA) were prepared by pulsed laser deposition (PLD). Using a KrF excimer laser beam focused on a target made of pressed powder with a fluence in the 1-2 J cm -2 range, films of about 1μm thickness and low roughness could be obtained on silica substrates at room temperature and under a pressure of about 10 -5 mbar. The Fourier transform infrared (FTIR) spectra of the films, recorded in attenuated total reflection (ATR) configuration, are nearly identical to those of the starting powders. For fluences lower than 0.6 J cm -2 , however, the structure of the molecule is not preserved. Atomic force microscopy (AFM) images show the protein to be deposited as particles of about 100 nm diameter without clear ordering and the films to have low roughness. Refractive index and thickness of the films were measured by m-lines spectroscopy.

KW - AFM

KW - FTIR

KW - Laser deposition

KW - Protein

KW - Thin films

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U2 - 10.1016/S0169-4332(02)01419-8

DO - 10.1016/S0169-4332(02)01419-8

M3 - Artículo

AN - SCOPUS:0037443269

SN - 0169-4332

VL - 208-209

SP - 658

EP - 662

JO - Applied Surface Science

JF - Applied Surface Science

IS - 1

ER -

Pulsed laser deposition of bovine serum albumin protein thin films

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Keywords

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