TY - JOUR
T1 - Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii
AU - Almaraz-Delgado, Alma Lorena
AU - Flores-Uribe, José
AU - Pérez-España, Víctor Hugo
AU - Salgado-Manjarrez, Edgar
AU - Badillo-Corona, Jesús Agustín
N1 - Publisher Copyright:
© 2014, Almaraz Delgado et al.; licensee Springer.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have been successfully expressed in the chloroplast using endogenous and chimeric promoter sequences. In some cases, proteins have accumulated to high level, demonstrating that this technology could compete with current production platforms. This review focuses on the works that have engineered the chloroplast of C. reinhardtii with the aim of producing recombinant proteins intended for therapeutical use in humans or animals.
AB - Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have been successfully expressed in the chloroplast using endogenous and chimeric promoter sequences. In some cases, proteins have accumulated to high level, demonstrating that this technology could compete with current production platforms. This review focuses on the works that have engineered the chloroplast of C. reinhardtii with the aim of producing recombinant proteins intended for therapeutical use in humans or animals.
KW - Chlamydomonas
KW - Chloroplast transformation
KW - Recombinant therapeutic proteins
UR - http://www.scopus.com/inward/record.url?scp=84929048742&partnerID=8YFLogxK
U2 - 10.1186/s13568-014-0057-4
DO - 10.1186/s13568-014-0057-4
M3 - Artículo
C2 - 25136510
SN - 2191-0855
VL - 4
SP - 1
EP - 9
JO - AMB Express
JF - AMB Express
IS - 1
M1 - 57
ER -