TY - JOUR
T1 - Production of penicillin acylase by a recombinant Escherichia coli using cheese whey as substrate and inducer
AU - De León-Rodríguez, Antonio
AU - Rivera-Pastrana, Dulce
AU - Medina-Rivero, Emilio
AU - Flores-Flores, José Luis
AU - Estrada-Baltazar, Alejandro
AU - Ordóñez-Acevedo, Leandro G.
AU - de la Rosa, Ana P.Barba
N1 - Funding Information:
This work was supported by CONACyT Grant no. 39639.
PY - 2006/12
Y1 - 2006/12
N2 - Cheese whey (CW) is the major subproduct from cheese manufacturing and it is considered as a waste pollutant since its high content of lactose. In this work a fermentation process for the production of penicillin acylase (PA) by a recombinant Escherichia coli and using CW as unique carbon source and inducer was developed. A design factorial 32 was used to evaluate the influence of independent variables (dissolved oxygen and CW concentration) on the ability of E. coli W3110/pPA102 to produce PA. Maximum specific PA activity of 781 U g-1 was attained at 5 g L-1 of CW and 3% dissolved oxygen. The results showed that CW can be used successfully as unique carbon source and inducer for the production of recombinant proteins using constructions driven by the lac promoter and this way reducing the discharges of that pollutant to the environment.
AB - Cheese whey (CW) is the major subproduct from cheese manufacturing and it is considered as a waste pollutant since its high content of lactose. In this work a fermentation process for the production of penicillin acylase (PA) by a recombinant Escherichia coli and using CW as unique carbon source and inducer was developed. A design factorial 32 was used to evaluate the influence of independent variables (dissolved oxygen and CW concentration) on the ability of E. coli W3110/pPA102 to produce PA. Maximum specific PA activity of 781 U g-1 was attained at 5 g L-1 of CW and 3% dissolved oxygen. The results showed that CW can be used successfully as unique carbon source and inducer for the production of recombinant proteins using constructions driven by the lac promoter and this way reducing the discharges of that pollutant to the environment.
KW - Batch processing
KW - Growth kinetics
KW - Lactose
KW - Protein
KW - Recombinant DNA
UR - http://www.scopus.com/inward/record.url?scp=33751204743&partnerID=8YFLogxK
U2 - 10.1016/j.bioeng.2006.09.003
DO - 10.1016/j.bioeng.2006.09.003
M3 - Artículo
SN - 1389-0344
VL - 23
SP - 299
EP - 305
JO - Biomolecular Engineering
JF - Biomolecular Engineering
IS - 6
ER -