TY - JOUR
T1 - Production of indole-3-acetic acid by Bacillus circulans E9 in a low-cost medium in a bioreactor
AU - Sarmiento-López, Luis Gerardo
AU - López-Meyer, Melina
AU - Maldonado-Mendoza, Ignacio Eduardo
AU - Quiroz-Figueroa, Francisco Roberto
AU - Sepúlveda-Jiménez, Gabriela
AU - Rodríguez-Monroy, Mario
N1 - Publisher Copyright:
© 2022 The Society for Biotechnology, Japan
PY - 2022/7
Y1 - 2022/7
N2 - Bacillus circulans E9 (now known as Niallia circulans) promotes plant growth-producing indole-3-acetic acid (IAA), showing potential for use as a biofertilizer. In this work, the use of a low-cost medium containing industrial substrates, soybean, pea flour, Solulys, Pharmamedia, yeast extract, and sodium chloride (NaCl), was evaluated as a substitute for microbiological Luria Broth (LB) medium for the growth of B. circulans E9 and the production of IAA. In Erlenmeyer flasks with pea fluor medium (PYM), the maximum production of IAA was 7.81 ± 0.16 μg mL−1, while in microbiological LB medium, it was 3.73 ± 0.15 μg mL−1. In addition, an oxygen transfer rate (OTR) of 1.04 kg O2 m−3 d−1 allowed the highest bacterial growth (19.3 ± 2.18 × 1010 CFU mL−1) and IAA production (10.7 μg mL−1). Consequently, the OTR value from the flask experiments was used to define the conditions for the operation of a 1 L stirred tank bioreactor. The growth and IAA production of B. circulans cultured in a bioreactor with PYM medium were higher (8 and 1.6 times, respectively) than those of bacteria cultured in Erlenmeyer flasks. IAA produced in a bioreactor by B. circulans was shown to induce the root system in Arabidopsis thaliana, similar to synthetic IAA. The results of this study demonstrate that PYM medium may be able to be used for the mass production of B. circulans E9 in bioreactors, increasing both bacterial growth and IAA production. This low-cost medium has the potential to be employed to grow other IAA-producing bacterial species.
AB - Bacillus circulans E9 (now known as Niallia circulans) promotes plant growth-producing indole-3-acetic acid (IAA), showing potential for use as a biofertilizer. In this work, the use of a low-cost medium containing industrial substrates, soybean, pea flour, Solulys, Pharmamedia, yeast extract, and sodium chloride (NaCl), was evaluated as a substitute for microbiological Luria Broth (LB) medium for the growth of B. circulans E9 and the production of IAA. In Erlenmeyer flasks with pea fluor medium (PYM), the maximum production of IAA was 7.81 ± 0.16 μg mL−1, while in microbiological LB medium, it was 3.73 ± 0.15 μg mL−1. In addition, an oxygen transfer rate (OTR) of 1.04 kg O2 m−3 d−1 allowed the highest bacterial growth (19.3 ± 2.18 × 1010 CFU mL−1) and IAA production (10.7 μg mL−1). Consequently, the OTR value from the flask experiments was used to define the conditions for the operation of a 1 L stirred tank bioreactor. The growth and IAA production of B. circulans cultured in a bioreactor with PYM medium were higher (8 and 1.6 times, respectively) than those of bacteria cultured in Erlenmeyer flasks. IAA produced in a bioreactor by B. circulans was shown to induce the root system in Arabidopsis thaliana, similar to synthetic IAA. The results of this study demonstrate that PYM medium may be able to be used for the mass production of B. circulans E9 in bioreactors, increasing both bacterial growth and IAA production. This low-cost medium has the potential to be employed to grow other IAA-producing bacterial species.
KW - Arabidopsis thaliana
KW - Indole-3-acetic acid
KW - Low-cost industrial substrates
KW - Oxygen transfer rate
KW - Stirred tank bioreactor
UR - http://www.scopus.com/inward/record.url?scp=85129106314&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2022.03.007
DO - 10.1016/j.jbiosc.2022.03.007
M3 - Artículo
C2 - 35461767
AN - SCOPUS:85129106314
SN - 1389-1723
VL - 134
SP - 21
EP - 28
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 1
ER -