TY - JOUR
T1 - Production of IL-10, TNF and IL-12 by Peripheral Blood Mononuclear Cells in Mexican Workers Exposed to a Mixture of Benzene-Toluene-Xylene
AU - Haro-García, Luis Cuauhtémoc
AU - Juárez-Pérez, Cuauhtémoc Arturo
AU - Aguilar-Madrid, Guadalupe
AU - Vélez-Zamora, Nadia Mayola
AU - Muñoz-Navarro, Sergio
AU - Chacón-Salinas, Rommel
AU - González-Bonilla, César Raúl
AU - Iturbe-Haro, Claudia Rosa
AU - Estrada-García, Iris
AU - Borja-Aburto, Víctor Hugo
N1 - Funding Information:
The study received financial support from the Consejo Nacional de Ciencia y Tecnología (CONACyT) through its Sectorial Fund for Research on Health and Social Security: 2004-C01-077 and CONACYT-C01-077; the Fondo del Fomento a la Investigación (FOFOI) of the Mexican Social Security Institute: 2005/1/I/162; and CONACyT, through its Integrated Support for the Training of Doctors of Science Program 2006: I0006-2006-01. The project also was supported by the Irving J. Selikoff International Scholar of the Mount Sinai School of Medicine Award Number D43TW000640 from the Fogarty International Center and by the Miguel Aleman Foundation 2003–2005.
PY - 2012/1
Y1 - 2012/1
N2 - Background and Aims: Occupational exposure to low-level benzene and the joint action of toluene-xylene probably cause effects on circulating monocytes immune response. We undertook this study to determine relationship between occupational exposure to benzene-toluene-xylene mixture (BTX) and IL-10, TNF and IL-12 production by peripheral blood mononuclear cells. Methods: Exposure was estimated in 54 workers from a paint company in Mexico City through BTX . accumulated potential dose (BTX-APD). Two exposure groups were formed: high and low BTX-APD established with a cutoff point at ≥1.0 of BTX-APD, as a function of the geometric mean of the estimator's value distribution and the higher agreement between BTX-APD ≥1.0 and the areas referred as using (or not) organic solvents in the work process. IL-10, TNF and IL-12 concentrations were measured with ELISA. Through multiple linear regression models, the production of each of the proposed cytokines and of the whole set was assessed. Results: Workers with high BTX-APD showed a significant reduction in TNF production (β = -1,196.0 pg/mL; . p = 0.01); a reduction for IL-10 (β = -520.3; . p = 0.13) and IL-12 (β = -843.3; . p = 0.09) was also observed, although without statistical significance. Conclusions: TNF production assessed in workers with a high BTX-APD is lower than in those with a low BTX-APD, but not in IL-10 and IL-12 production.
AB - Background and Aims: Occupational exposure to low-level benzene and the joint action of toluene-xylene probably cause effects on circulating monocytes immune response. We undertook this study to determine relationship between occupational exposure to benzene-toluene-xylene mixture (BTX) and IL-10, TNF and IL-12 production by peripheral blood mononuclear cells. Methods: Exposure was estimated in 54 workers from a paint company in Mexico City through BTX . accumulated potential dose (BTX-APD). Two exposure groups were formed: high and low BTX-APD established with a cutoff point at ≥1.0 of BTX-APD, as a function of the geometric mean of the estimator's value distribution and the higher agreement between BTX-APD ≥1.0 and the areas referred as using (or not) organic solvents in the work process. IL-10, TNF and IL-12 concentrations were measured with ELISA. Through multiple linear regression models, the production of each of the proposed cytokines and of the whole set was assessed. Results: Workers with high BTX-APD showed a significant reduction in TNF production (β = -1,196.0 pg/mL; . p = 0.01); a reduction for IL-10 (β = -520.3; . p = 0.13) and IL-12 (β = -843.3; . p = 0.09) was also observed, although without statistical significance. Conclusions: TNF production assessed in workers with a high BTX-APD is lower than in those with a low BTX-APD, but not in IL-10 and IL-12 production.
KW - Benzene-Toluene-Xylene
KW - Cytokines
KW - Immune response
KW - Occupational exposure
UR - http://www.scopus.com/inward/record.url?scp=84859260847&partnerID=8YFLogxK
U2 - 10.1016/j.arcmed.2012.01.008
DO - 10.1016/j.arcmed.2012.01.008
M3 - Artículo
C2 - 22300681
SN - 0188-4409
VL - 43
SP - 51
EP - 57
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 1
ER -