TY - JOUR
T1 - Production of dihydroxylated betalains and dopamine in cell suspension cultures of celosia argentea var. Plumosa
AU - Guadarrama-Flores, Berenice
AU - Rodriguez-Monroy, Mario
AU - Cruz-Sosa, Francisco
AU - Garcia-Carmona, Francisco
AU - Gandia-Herrero, Fernando
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/3/18
Y1 - 2015/3/18
N2 - Betalains are plant pigments of hydrophilic nature with demonstrated chemopreventive potential in cancer cell lines and animal models. Among the betalains, those containing an aromatic moiety with two free hydroxyl groups possess the strongest antioxidant and free radical scavenging activities. The betaxanthins dopaxanthin and miraxanthin V and the betacyanins betanidin and decarboxy-betanidin are the only natural betalains with catecholic substructures. These four pigments have been produced in cell cultures established from hypocotyls of the plant Celosia argentea. Two stable and differentially colored cell lines, yellow and red, were maintained on Murashige and Skoog medium supplemented with the plant growth regulators 6-benzylaminopurine (6.66 μM) and 2,4-dichlorophenoxyacetic acid (6.79 μM). Derived suspension cultures showed increased production of dihydroxylated betalains in the cells and secreted to the medium with a maximum reached after 8 days of culture. In addition, precursor molecules betalamic acid and dopamine, with content up to 42.08 mg/g dry weight, were also obtained. The joint presence of the bioactive betalains together with the production of dopamine and betalamic acid show the ability of cell cultures of C. argentea to become a stable source of valuable phytochemicals.
AB - Betalains are plant pigments of hydrophilic nature with demonstrated chemopreventive potential in cancer cell lines and animal models. Among the betalains, those containing an aromatic moiety with two free hydroxyl groups possess the strongest antioxidant and free radical scavenging activities. The betaxanthins dopaxanthin and miraxanthin V and the betacyanins betanidin and decarboxy-betanidin are the only natural betalains with catecholic substructures. These four pigments have been produced in cell cultures established from hypocotyls of the plant Celosia argentea. Two stable and differentially colored cell lines, yellow and red, were maintained on Murashige and Skoog medium supplemented with the plant growth regulators 6-benzylaminopurine (6.66 μM) and 2,4-dichlorophenoxyacetic acid (6.79 μM). Derived suspension cultures showed increased production of dihydroxylated betalains in the cells and secreted to the medium with a maximum reached after 8 days of culture. In addition, precursor molecules betalamic acid and dopamine, with content up to 42.08 mg/g dry weight, were also obtained. The joint presence of the bioactive betalains together with the production of dopamine and betalamic acid show the ability of cell cultures of C. argentea to become a stable source of valuable phytochemicals.
KW - antiradical
KW - betalains
KW - bioactive
KW - dopamine
KW - secondary metabolism
UR - http://www.scopus.com/inward/record.url?scp=84924939704&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b00065
DO - 10.1021/acs.jafc.5b00065
M3 - Artículo
C2 - 25727687
SN - 0021-8561
VL - 63
SP - 2741
EP - 2749
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 10
ER -