TY - JOUR
T1 - Production of 6-pentyl-α-pyrone by Trichoderma harzianum cultured in unbaffled and baffled shake flasks
AU - Galindo, Enrique
AU - Flores, Celia
AU - Larralde-Corona, Patricia
AU - Corkidi-Blanco, Gabriel
AU - Rocha-Valadez, J. Antonio
AU - Serrano-Carreón, Leobardo
N1 - Funding Information:
The authors would like to thank the financial support of CONACyT (grant 39906), DGAPA (grant IN 226202-2) and CONACyT-IFS (grant E2548-2). We thank the technical support of L. Vega-Alvarado in the image analysis work and M. Estrada-Estrada in some of the experiments. We thank Prof. J. Büchs for the access to his actual data for calculating volumetric power drawn in unbaffled shake flasks as well as for critically reading the manuscript.
PY - 2004/4
Y1 - 2004/4
N2 - When Trichoderma harzianum was cultured in unbaffled shake flasks, increased agitation (100-300rpm) resulted in higher biomass production and higher titers of 6-pentyl-α-pyrone (up to 100mg/l). The fungus grew as loose aggregates, being smaller at the highest agitation rate. In baffled flasks, loose aggregates were only observed at the lowest agitation speed (100rpm). At higher speeds, nearly 90% of the total biomass was attached on the surface of the flask' wall. The remaining biomass (suspended in the liquid) was in the form of macroscopic pellets. 6-Pentyl-α-pyrone (6-PP) titer in baffled flasks cultures was maximum (96mg/l) at 100rpm and decreased at higher agitation rates. The data suggest that specific 6-PP productivity was determined by hydrodynamic stress, reaching a maximum for an estimated volumetric power drawn of about 0.9kW/m3 and then dropping at higher volumetric power drawn.
AB - When Trichoderma harzianum was cultured in unbaffled shake flasks, increased agitation (100-300rpm) resulted in higher biomass production and higher titers of 6-pentyl-α-pyrone (up to 100mg/l). The fungus grew as loose aggregates, being smaller at the highest agitation rate. In baffled flasks, loose aggregates were only observed at the lowest agitation speed (100rpm). At higher speeds, nearly 90% of the total biomass was attached on the surface of the flask' wall. The remaining biomass (suspended in the liquid) was in the form of macroscopic pellets. 6-Pentyl-α-pyrone (6-PP) titer in baffled flasks cultures was maximum (96mg/l) at 100rpm and decreased at higher agitation rates. The data suggest that specific 6-PP productivity was determined by hydrodynamic stress, reaching a maximum for an estimated volumetric power drawn of about 0.9kW/m3 and then dropping at higher volumetric power drawn.
KW - Agitation
KW - Biosynthesis
KW - Filamentous fungi
KW - Mixing
KW - Submerged culture
UR - http://www.scopus.com/inward/record.url?scp=1542268885&partnerID=8YFLogxK
U2 - 10.1016/S1369-703X(03)00115-3
DO - 10.1016/S1369-703X(03)00115-3
M3 - Artículo
SN - 1369-703X
VL - 18
SP - 1
EP - 8
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
IS - 1
ER -