TY - JOUR
T1 - Prickly pear polygalacturonase gene
T2 - cDNA cloning and transcript accumulation during ethylene treatment, cold storage and wounding
AU - de Fátima Rosas-Cárdenas, Flor
AU - Valderrama-Cháirez, María Leonor
AU - Cruz-Hernández, Andrés
AU - Paredes-López, Octavio
N1 - Funding Information:
We acknowledge financial support from SIHGO-CONACyT, CONCyTEG, and Fundación Guanajuato Produce A.C. Thanks to Dr. Candelario Mondragón (INIFAP) for assistance in the experimental orchard and to Dr. Edmundo Mercado (UAQ) for helping in the ethylene treatments. Technical assistance by Drs. María E. Valverde and Fidel Guevara is also acknowledged.
PY - 2007/6
Y1 - 2007/6
N2 - Polygalacturonase (PG; EC 3.2.1.6.9) has been the most widely studied cell wall hydrolase in fruit ripening. Degenerate oligonucleotides corresponding to conserved regions from reported sequences were used as primers for RT-PCR to amplify mRNA extracted from middle ripe fruit. Cloning and characterization of a cDNA OsPG showed a 282 bp product with a predicted sequence of 94 amino acids. The peptide exhibited a high identity with previously reported fruit PGs. Northern blot analysis of the messenger showed a 1.7 kb transcript induced during prickly pear ripening. It was found by Southern blot analysis that there is one copy of this gene. The OsPG mRNA expression is sensitive to ethylene, cold storage and wounding.
AB - Polygalacturonase (PG; EC 3.2.1.6.9) has been the most widely studied cell wall hydrolase in fruit ripening. Degenerate oligonucleotides corresponding to conserved regions from reported sequences were used as primers for RT-PCR to amplify mRNA extracted from middle ripe fruit. Cloning and characterization of a cDNA OsPG showed a 282 bp product with a predicted sequence of 94 amino acids. The peptide exhibited a high identity with previously reported fruit PGs. Northern blot analysis of the messenger showed a 1.7 kb transcript induced during prickly pear ripening. It was found by Southern blot analysis that there is one copy of this gene. The OsPG mRNA expression is sensitive to ethylene, cold storage and wounding.
KW - Cell wall
KW - Fruit ripening
KW - Non-climacteric
KW - Polygalacturonase
KW - Prickly pear
KW - Softening
UR - http://www.scopus.com/inward/record.url?scp=34047192625&partnerID=8YFLogxK
U2 - 10.1016/j.postharvbio.2006.12.008
DO - 10.1016/j.postharvbio.2006.12.008
M3 - Artículo
SN - 0925-5214
VL - 44
SP - 254
EP - 259
JO - Postharvest Biology and Technology
JF - Postharvest Biology and Technology
IS - 3
ER -