TY - JOUR
T1 - Prevalence and characterization of Listeria monocytogenes isolated from pork meat and on inert surfaces
AU - Figueroa-López, Alejandro Miguel
AU - Maldonado-Mendoza, Ignacio Eduardo
AU - López-Cervantes, Jaime
AU - Verdugo-Fuentes, Abel Alberto
AU - Ruiz-Vega, Dante Alejandro
AU - Cantú-Soto, Ernesto Uriel
N1 - Publisher Copyright:
© 2019, Sociedade Brasileira de Microbiologia.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - This study focuses on the prevalence of Listeria monocytogenes (Lm) in pork meat and on inert surfaces from slaughterhouses in Sonora, Mexico. A total of 21 Lm were obtained from 103 samples, giving a prevalence of 20.3%. The prevalence of Lm in pork loin was 15.9% and 20.8% for inert surfaces in Federal Inspection Type (FIT) slaughterhouses. For non-FIT slaughterhouses, the prevalence was 25.7%. PCR amplification of genomic DNA from the Lm isolates revealed the presence of the hlyA gene, suggesting a pathogenic nature for these isolates. The isolates obtained in this work all clustered with Lm, according to our phylogenetic analysis based on the 16S rDNA sequence. This Lm cluster indicates that Lm isolates 7-2, 4, 2-1, 10B, 8, 3, 3-3, and 9 share 16S rRNA identity with other Lm isolates that have been reported as foodborne pathogens (rR2-502, J1817, J1816, J1926) and that are involved in foodborne outbreaks. The most commonly detected serotypes were 1/2a and 1/2b. All isolates displayed differential responses to the assayed antibiotics, and most isolates were able to grow in the presence of penicillin G, or both penicillin and penicillin-derived (oxacillin) antibiotics.
AB - This study focuses on the prevalence of Listeria monocytogenes (Lm) in pork meat and on inert surfaces from slaughterhouses in Sonora, Mexico. A total of 21 Lm were obtained from 103 samples, giving a prevalence of 20.3%. The prevalence of Lm in pork loin was 15.9% and 20.8% for inert surfaces in Federal Inspection Type (FIT) slaughterhouses. For non-FIT slaughterhouses, the prevalence was 25.7%. PCR amplification of genomic DNA from the Lm isolates revealed the presence of the hlyA gene, suggesting a pathogenic nature for these isolates. The isolates obtained in this work all clustered with Lm, according to our phylogenetic analysis based on the 16S rDNA sequence. This Lm cluster indicates that Lm isolates 7-2, 4, 2-1, 10B, 8, 3, 3-3, and 9 share 16S rRNA identity with other Lm isolates that have been reported as foodborne pathogens (rR2-502, J1817, J1816, J1926) and that are involved in foodborne outbreaks. The most commonly detected serotypes were 1/2a and 1/2b. All isolates displayed differential responses to the assayed antibiotics, and most isolates were able to grow in the presence of penicillin G, or both penicillin and penicillin-derived (oxacillin) antibiotics.
KW - Antibiotics
KW - Listeria monocytogenes
KW - Pathogen occurrence
KW - Pork meat
UR - http://www.scopus.com/inward/record.url?scp=85064923960&partnerID=8YFLogxK
U2 - 10.1007/s42770-019-00073-7
DO - 10.1007/s42770-019-00073-7
M3 - Artículo
C2 - 30976991
AN - SCOPUS:85064923960
SN - 1517-8382
VL - 50
SP - 817
EP - 824
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
IS - 3
ER -