TY - JOUR
T1 - Presence of indicator bacteria, diarrhoeagenic Escherichia coli pathotypes and Salmonella in fresh carrot juice from Mexican restaurants
AU - Torres-Vitela, M. D.R.
AU - Gómez Aldapa, C. A.
AU - Cerna-Cortes, J. F.
AU - Villarruel-López, A.
AU - Rangel-Vargas, E.
AU - Castro-Rosas, J.
N1 - Funding Information:
The work was supported by National Institutes of Health grant R01GM120043 and National Science Foundation Eager grant MCB1747705 to H.R. Kaback, as well as National Institutes of Health grants R01GM122759 and R21NS105863 to L. Guan. H.R. Kaback also gratefully acknowledges support from the
Funding Information:
The work was supported by National Institutes of Health grant R01GM120043 and National Science Foundation Eager grant MCB1747705 to H.R. Kaback, as well as National Institutes of Health grants R01GM122759 and R21NS105863 to L. Guan. H.R. Kaback also gratefully acknowledges support from the University of California, Los Angeles, and a gift from Ruth and Bucky Stein.
PY - 2013/3
Y1 - 2013/3
N2 - Coliform bacteria (CB), faecal coliforms (FC), Escherichia coli, diarrhoeagenic E.coli pathotypes (DEP) and Salmonella frequencies were determined for fresh carrot juice from restaurants in Pachuca city, Mexico. Two hundred and eighty carrot juice samples were purchased in three types of restaurants: (A), national chain restaurants; (B), local restaurants; and (C), very small restaurants. Two restaurants for each A and B, and three for C, were included. Forty juice samples were purchased at each restaurant. All tested juice samples had poor microbiological quality. Of these samples, 100, 96·8, 54·3, 8·9 and 8·6% had CB, FC, E.coli, DEP and Salmonella, respectively. CB were present in all juice samples regardless of source, with limits ranging from 3·6×102 to 8·5×107 CFU ml-1, and the limits for FC and E.coli were <3 to 1100 MPN ml-1 and <3 to 460 MPN, respectively. DEP and Salmonella were isolated from samples from all the restaurants at levels of 5% or above: DEP, 5% (A1, B2), 10% (A2, B1, C1, C2) and 12·5% (C3); Salmonella, 5% (A1, A2, B2), 7·5% (C2), 10% (C1), 12·5% (B1) and 15% (C3). Significance and Impact of the Study: This is the first report of microbiological quality and Salmonella, enteroinvasive E.coli (EIEC), enterotoxigenic E.coli (ETEC) and Shiga toxin-producing E.coli (STEC) isolation from fresh carrot juice in Mexico. Fresh carrot juice from restaurants could be an important factor contributing to the endemicity of EIEC-, ETEC- and STEC- and Salmonella-caused gastroenteritis in Mexico.
AB - Coliform bacteria (CB), faecal coliforms (FC), Escherichia coli, diarrhoeagenic E.coli pathotypes (DEP) and Salmonella frequencies were determined for fresh carrot juice from restaurants in Pachuca city, Mexico. Two hundred and eighty carrot juice samples were purchased in three types of restaurants: (A), national chain restaurants; (B), local restaurants; and (C), very small restaurants. Two restaurants for each A and B, and three for C, were included. Forty juice samples were purchased at each restaurant. All tested juice samples had poor microbiological quality. Of these samples, 100, 96·8, 54·3, 8·9 and 8·6% had CB, FC, E.coli, DEP and Salmonella, respectively. CB were present in all juice samples regardless of source, with limits ranging from 3·6×102 to 8·5×107 CFU ml-1, and the limits for FC and E.coli were <3 to 1100 MPN ml-1 and <3 to 460 MPN, respectively. DEP and Salmonella were isolated from samples from all the restaurants at levels of 5% or above: DEP, 5% (A1, B2), 10% (A2, B1, C1, C2) and 12·5% (C3); Salmonella, 5% (A1, A2, B2), 7·5% (C2), 10% (C1), 12·5% (B1) and 15% (C3). Significance and Impact of the Study: This is the first report of microbiological quality and Salmonella, enteroinvasive E.coli (EIEC), enterotoxigenic E.coli (ETEC) and Shiga toxin-producing E.coli (STEC) isolation from fresh carrot juice in Mexico. Fresh carrot juice from restaurants could be an important factor contributing to the endemicity of EIEC-, ETEC- and STEC- and Salmonella-caused gastroenteritis in Mexico.
KW - Diarrhoeagenic E.coli pathotypes
KW - EIEC
KW - ETEC
KW - Fresh carrot juice
KW - Restaurant
KW - STEC
KW - Salmonella
UR - http://www.scopus.com/inward/record.url?scp=84874118742&partnerID=8YFLogxK
U2 - 10.1111/lam.12030
DO - 10.1111/lam.12030
M3 - Artículo
C2 - 23199003
SN - 0266-8254
VL - 56
SP - 180
EP - 185
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 3
ER -