TY - JOUR
T1 - Presence of benzoate type toxins in Gymnodinium catenatum Graham isolated from the Mexican Pacific
AU - Bustillos-Guzmán, J.
AU - Vale, P.
AU - Band-Schmidt, C.
N1 - Funding Information:
We thank to Francisco Hernández-Sandoval for his help in the extraction of G. catenatum cells. Funding for this project was provided by projects SIP 20100192, SEP-CONACyT 61226 and PC3.1 from CIBNOR , and IPIMAR funding.
PY - 2011/5
Y1 - 2011/5
N2 - Benzoate type toxins have been described as an important component of Gymnodinium catenatum cells. In this paper we study these toxins in a G. catenatum strain isolated from the Mexican coast. A partition of the toxins was done by solid-phase extraction on a COOH cartridge and detected by HPLC coupled to fluorescence after pre-column periodate oxidation. Two groups of the hydrophobic analogues of saxitoxin were identified: those containing a sulphate group in the benzoate moiety instead of a hydroxyl group like GC1/2 or GC3 and the hydroxy-benzoate analogues, with a sulphate group at the eleventh position of the STX core present or absent (GCs-GTX and GCs-STX analogues, respectively). These toxins are more abundant, in a relative basis, when comparing with a G. catenatum toxin content isolated from Portugal. This is the first report of the presence of these toxins in a Mexican strain.
AB - Benzoate type toxins have been described as an important component of Gymnodinium catenatum cells. In this paper we study these toxins in a G. catenatum strain isolated from the Mexican coast. A partition of the toxins was done by solid-phase extraction on a COOH cartridge and detected by HPLC coupled to fluorescence after pre-column periodate oxidation. Two groups of the hydrophobic analogues of saxitoxin were identified: those containing a sulphate group in the benzoate moiety instead of a hydroxyl group like GC1/2 or GC3 and the hydroxy-benzoate analogues, with a sulphate group at the eleventh position of the STX core present or absent (GCs-GTX and GCs-STX analogues, respectively). These toxins are more abundant, in a relative basis, when comparing with a G. catenatum toxin content isolated from Portugal. This is the first report of the presence of these toxins in a Mexican strain.
KW - Benzoate toxins
KW - Gymnodinium catenatum
KW - HPLC
KW - Mexico
UR - http://www.scopus.com/inward/record.url?scp=79955020711&partnerID=8YFLogxK
U2 - 10.1016/j.toxicon.2011.02.023
DO - 10.1016/j.toxicon.2011.02.023
M3 - Artículo
C2 - 21376072
SN - 0041-0101
VL - 57
SP - 922
EP - 926
JO - Toxicon
JF - Toxicon
IS - 6
ER -