Preparación y evaluación del reactivo de coaglutinación para la identificación de Vibrio cholerae 01. Su ensayo durante un brote de cólera en Minatitlán, Veracruz.

C. González Bonilla; A. Villanueva Zamudio; G. Bolaños Monrroy; S. Giono Cerezo; J. L. Valdespino Gómez

Preparación y evaluación del reactivo de coaglutinación para la identificación de Vibrio cholerae 01. Su ensayo durante un brote de cólera en Minatitlán, Veracruz.

Translated title of the contribution: Preparation and evaluation of coagglutination reagents for the identification of Vibrio cholerae 01. Its trial during a cholera outbreak in Minatitlán, Veracruz

C. González Bonilla, A. Villanueva Zamudio, G. Bolaños Monrroy, S. Giono Cerezo, J. L. Valdespino Gómez

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

Abstract

This study was realized in Minatitlán, Veracruz during a cholera outbreak. 169 rectal swabs were taken from hilles and their contacts. They were transfer alkaline peptone water for enrichment to V. cholerae and incubated for 8 hrs to 37 degrees C, 70 were positive for V. cholerae in both techniques. The coagglutination was done with a reagent prepared at the Instituto de Diagnóstico y Referencia Epidemiológicos of México and the culture were also performed in the same Institute. We obtained 100% of sensitivity and specificity of co-agglutination in relation with culture. This results gave the possibility to use this kind or reagents for a rapid presumptive diagnosis of cholera.

Translated title of the contribution	Preparation and evaluation of coagglutination reagents for the identification of Vibrio cholerae 01. Its trial during a cholera outbreak in Minatitlán, Veracruz
Original language	Spanish
Pages (from-to)	153-155
Number of pages	3
Journal	Revista latinoamericana de microbiologia
Volume	34
Issue number	2
State	Published - Apr 1992

Cite this

@article{12553ccc3a3b491f9f557a98466c04eb,

title = "Preparaci{\'o}n y evaluaci{\'o}n del reactivo de coaglutinaci{\'o}n para la identificaci{\'o}n de Vibrio cholerae 01. Su ensayo durante un brote de c{\'o}lera en Minatitl{\'a}n, Veracruz.",

abstract = "This study was realized in Minatitl{\'a}n, Veracruz during a cholera outbreak. 169 rectal swabs were taken from hilles and their contacts. They were transfer alkaline peptone water for enrichment to V. cholerae and incubated for 8 hrs to 37 degrees C, 70 were positive for V. cholerae in both techniques. The coagglutination was done with a reagent prepared at the Instituto de Diagn{\'o}stico y Referencia Epidemiol{\'o}gicos of M{\'e}xico and the culture were also performed in the same Institute. We obtained 100% of sensitivity and specificity of co-agglutination in relation with culture. This results gave the possibility to use this kind or reagents for a rapid presumptive diagnosis of cholera.",

author = "{Gonz{\'a}lez Bonilla}, C. and {Villanueva Zamudio}, A. and {Bola{\~n}os Monrroy}, G. and {Giono Cerezo}, S. and {Valdespino G{\'o}mez}, {J. L.}",

year = "1992",

month = apr,

language = "Espa{\~n}ol",

volume = "34",

pages = "153--155",

journal = "Revista latinoamericana de microbiologia",

issn = "0187-4640",

number = "2",

}

Preparación y evaluación del reactivo de coaglutinación para la identificación de Vibrio cholerae 01. Su ensayo durante un brote de cólera en Minatitlán, Veracruz. / González Bonilla, C.; Villanueva Zamudio, A.; Bolaños Monrroy, G. et al.
In: Revista latinoamericana de microbiologia, Vol. 34, No. 2, 04.1992, p. 153-155.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Preparación y evaluación del reactivo de coaglutinación para la identificación de Vibrio cholerae 01. Su ensayo durante un brote de cólera en Minatitlán, Veracruz.

AU - González Bonilla, C.

AU - Villanueva Zamudio, A.

AU - Bolaños Monrroy, G.

AU - Giono Cerezo, S.

AU - Valdespino Gómez, J. L.

PY - 1992/4

Y1 - 1992/4

N2 - This study was realized in Minatitlán, Veracruz during a cholera outbreak. 169 rectal swabs were taken from hilles and their contacts. They were transfer alkaline peptone water for enrichment to V. cholerae and incubated for 8 hrs to 37 degrees C, 70 were positive for V. cholerae in both techniques. The coagglutination was done with a reagent prepared at the Instituto de Diagnóstico y Referencia Epidemiológicos of México and the culture were also performed in the same Institute. We obtained 100% of sensitivity and specificity of co-agglutination in relation with culture. This results gave the possibility to use this kind or reagents for a rapid presumptive diagnosis of cholera.

AB - This study was realized in Minatitlán, Veracruz during a cholera outbreak. 169 rectal swabs were taken from hilles and their contacts. They were transfer alkaline peptone water for enrichment to V. cholerae and incubated for 8 hrs to 37 degrees C, 70 were positive for V. cholerae in both techniques. The coagglutination was done with a reagent prepared at the Instituto de Diagnóstico y Referencia Epidemiológicos of México and the culture were also performed in the same Institute. We obtained 100% of sensitivity and specificity of co-agglutination in relation with culture. This results gave the possibility to use this kind or reagents for a rapid presumptive diagnosis of cholera.

UR - http://www.scopus.com/inward/record.url?scp=0026849673&partnerID=8YFLogxK

M3 - Artículo

SN - 0187-4640

VL - 34

SP - 153

EP - 155

JO - Revista latinoamericana de microbiologia

JF - Revista latinoamericana de microbiologia

IS - 2

ER -

Preparación y evaluación del reactivo de coaglutinación para la identificación de Vibrio cholerae 01. Su ensayo durante un brote de cólera en Minatitlán, Veracruz.

Abstract

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