Possible mode of emergence for drug-resistant leprosy is revealed by an analysis of samples from Mexico

Masanori Matsuoka, Yasuhiko Suzuki, Iris Estrada Garcia, Mary Fafutis-Morris, Alberto Vargas-Gonzaález, Cristina Carrenão-Martinez, Yukari Fukushima, Chie Nakajima

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18 Citations (Scopus)

Abstract

Mexico is a country with sporadic leprosy cases, and the reemergence of drug resistance is a concern. In this study, molecular analysis of Mycobacterium leprae was employed to clarify the spread of drug-resistant leprosy. Thus, drug resistance-determining regions in the folP1, rpoB, and gyrA genes, which are associated with resistance to dapsone, rifampicin, and ofloxacin, respectively, were analyzed by direct sequencing of the PCR product. No mutations in the folP1 gene were observed in any of the 72 slit skin samples obtained from 38 patients, although two samples carrying a mutation at codon 425 in the rpoB gene, which confers resistance to rifampicin, a key component of multidrug therapy, were identified. In addition, a mutation at codon 91 in the gyrA gene, which correlates with ofloxacin resistance, was found in one sample. These results demonstrate the existence of rifampicin- and ofloxacin- resistant leprosy. Interestingly, wild-type and mutant sequences in the gyrA gene were found to coexist in one clinical sample. In addition, all three drug resistance-related mutations were found in only one of the two earlobes of the patients concerned, suggesting a possible pathway for the spread of drug-resistant M. leprae.
Original languageAmerican English
Pages (from-to)412-416
Number of pages370
JournalJapanese Journal of Infectious Diseases
StatePublished - 6 Dec 2010

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Leprosy
Mexico
Ofloxacin
Rifampin
Drug Resistance
Mycobacterium leprae
Mutation
Pharmaceutical Preparations
Genes
Codon
Dapsone
Polymerase Chain Reaction
Skin

Cite this

Matsuoka, M., Suzuki, Y., Garcia, I. E., Fafutis-Morris, M., Vargas-Gonzaález, A., Carrenão-Martinez, C., ... Nakajima, C. (2010). Possible mode of emergence for drug-resistant leprosy is revealed by an analysis of samples from Mexico. Japanese Journal of Infectious Diseases, 412-416.
Matsuoka, Masanori ; Suzuki, Yasuhiko ; Garcia, Iris Estrada ; Fafutis-Morris, Mary ; Vargas-Gonzaález, Alberto ; Carrenão-Martinez, Cristina ; Fukushima, Yukari ; Nakajima, Chie. / Possible mode of emergence for drug-resistant leprosy is revealed by an analysis of samples from Mexico. In: Japanese Journal of Infectious Diseases. 2010 ; pp. 412-416.
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abstract = "Mexico is a country with sporadic leprosy cases, and the reemergence of drug resistance is a concern. In this study, molecular analysis of Mycobacterium leprae was employed to clarify the spread of drug-resistant leprosy. Thus, drug resistance-determining regions in the folP1, rpoB, and gyrA genes, which are associated with resistance to dapsone, rifampicin, and ofloxacin, respectively, were analyzed by direct sequencing of the PCR product. No mutations in the folP1 gene were observed in any of the 72 slit skin samples obtained from 38 patients, although two samples carrying a mutation at codon 425 in the rpoB gene, which confers resistance to rifampicin, a key component of multidrug therapy, were identified. In addition, a mutation at codon 91 in the gyrA gene, which correlates with ofloxacin resistance, was found in one sample. These results demonstrate the existence of rifampicin- and ofloxacin- resistant leprosy. Interestingly, wild-type and mutant sequences in the gyrA gene were found to coexist in one clinical sample. In addition, all three drug resistance-related mutations were found in only one of the two earlobes of the patients concerned, suggesting a possible pathway for the spread of drug-resistant M. leprae.",
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Matsuoka, M, Suzuki, Y, Garcia, IE, Fafutis-Morris, M, Vargas-Gonzaález, A, Carrenão-Martinez, C, Fukushima, Y & Nakajima, C 2010, 'Possible mode of emergence for drug-resistant leprosy is revealed by an analysis of samples from Mexico', Japanese Journal of Infectious Diseases, pp. 412-416.

Possible mode of emergence for drug-resistant leprosy is revealed by an analysis of samples from Mexico. / Matsuoka, Masanori; Suzuki, Yasuhiko; Garcia, Iris Estrada; Fafutis-Morris, Mary; Vargas-Gonzaález, Alberto; Carrenão-Martinez, Cristina; Fukushima, Yukari; Nakajima, Chie.

In: Japanese Journal of Infectious Diseases, 06.12.2010, p. 412-416.

Research output: Contribution to journalArticle

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AU - Matsuoka, Masanori

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Matsuoka M, Suzuki Y, Garcia IE, Fafutis-Morris M, Vargas-Gonzaález A, Carrenão-Martinez C et al. Possible mode of emergence for drug-resistant leprosy is revealed by an analysis of samples from Mexico. Japanese Journal of Infectious Diseases. 2010 Dec 6;412-416.