Plastid analysis of pigmented undifferentiated cells of marigold Tagetes erecta L. by transmission electron microscopy

Pablo Emilio Vanegas-Espinoza, Verónica Ramos-Viveros, Antonio Ruperto Jiménez-Aparicio, Oliver López-Villegas, Francisco José Heredia-Mira, Antonio Jesús Meléndez-Martínez, Adrián Guillermo Quintero-Gutiérrez, Octavio Paredes-López, Alma Angélica Del Villar-Martínez

Research output: Contribution to journalArticle

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Abstract

Marigold (Tagetes erecta) flowers are primarily used in industry for their high pigment content. Flower color development implies that chloroplast-chromoplast transition is associated with carotenoid biosynthesis. We report the recovery of undifferentiated pigmented marigold cells, various callus tissues, and their analysis by transmission electron microscopy in order to observe accumulating pigment and development of subcellular structures. Callus was generated from leaf explants and after several rounds of recurrent selection. Green-, yellow-, and brown-colored callus were obtained that showed distinct carotenoid profiles. For green material, violaxanthin, lutein, zeaxanthin, and β-carotene were produced, while yellow callus generated mainly lutein, as did the brown callus. Chloroplast-chromoplast transition was followed by measuring plastid size and shape in undifferentiated marigold cells by digital image analysis. Cellular alterations were evident in brown callus. Chloroplasts were the main structure in green callus, while yellow callus clearly showed the formation of plastoglobules, structures that are correlated with chloroplast-chromoplast transition. The high number of plastoglobules observed in yellow callus is possibly directly related to pigment synthesis and accumulation. © 2011 The Society for In Vitro Biology.
Original languageAmerican English
Pages (from-to)596-603
Number of pages535
JournalIn Vitro Cellular and Developmental Biology - Plant
DOIs
StatePublished - 1 Oct 2011

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plastids
Tagetes
chloroplasts
Tagetes erecta
Plastids
Bony Callus
Transmission Electron Microscopy
transmission electron microscopy
callus
pigments
carotenoids
cells
Chloroplasts
chromoplasts
Carotenoids
carotene
biosynthesis
Lutein
image analysis
leaves

Cite this

@article{11359aa1b55149a398fcda5fb3b3353f,
title = "Plastid analysis of pigmented undifferentiated cells of marigold Tagetes erecta L. by transmission electron microscopy",
abstract = "Marigold (Tagetes erecta) flowers are primarily used in industry for their high pigment content. Flower color development implies that chloroplast-chromoplast transition is associated with carotenoid biosynthesis. We report the recovery of undifferentiated pigmented marigold cells, various callus tissues, and their analysis by transmission electron microscopy in order to observe accumulating pigment and development of subcellular structures. Callus was generated from leaf explants and after several rounds of recurrent selection. Green-, yellow-, and brown-colored callus were obtained that showed distinct carotenoid profiles. For green material, violaxanthin, lutein, zeaxanthin, and β-carotene were produced, while yellow callus generated mainly lutein, as did the brown callus. Chloroplast-chromoplast transition was followed by measuring plastid size and shape in undifferentiated marigold cells by digital image analysis. Cellular alterations were evident in brown callus. Chloroplasts were the main structure in green callus, while yellow callus clearly showed the formation of plastoglobules, structures that are correlated with chloroplast-chromoplast transition. The high number of plastoglobules observed in yellow callus is possibly directly related to pigment synthesis and accumulation. {\circledC} 2011 The Society for In Vitro Biology.",
author = "Vanegas-Espinoza, {Pablo Emilio} and Ver{\'o}nica Ramos-Viveros and Jim{\'e}nez-Aparicio, {Antonio Ruperto} and Oliver L{\'o}pez-Villegas and Heredia-Mira, {Francisco Jos{\'e}} and Mel{\'e}ndez-Mart{\'i}nez, {Antonio Jes{\'u}s} and Quintero-Guti{\'e}rrez, {Adri{\'a}n Guillermo} and Octavio Paredes-L{\'o}pez and {Del Villar-Mart{\'i}nez}, {Alma Ang{\'e}lica}",
year = "2011",
month = "10",
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doi = "10.1007/s11627-011-9401-4",
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Plastid analysis of pigmented undifferentiated cells of marigold Tagetes erecta L. by transmission electron microscopy. / Vanegas-Espinoza, Pablo Emilio; Ramos-Viveros, Verónica; Jiménez-Aparicio, Antonio Ruperto; López-Villegas, Oliver; Heredia-Mira, Francisco José; Meléndez-Martínez, Antonio Jesús; Quintero-Gutiérrez, Adrián Guillermo; Paredes-López, Octavio; Del Villar-Martínez, Alma Angélica.

In: In Vitro Cellular and Developmental Biology - Plant, 01.10.2011, p. 596-603.

Research output: Contribution to journalArticle

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T1 - Plastid analysis of pigmented undifferentiated cells of marigold Tagetes erecta L. by transmission electron microscopy

AU - Vanegas-Espinoza, Pablo Emilio

AU - Ramos-Viveros, Verónica

AU - Jiménez-Aparicio, Antonio Ruperto

AU - López-Villegas, Oliver

AU - Heredia-Mira, Francisco José

AU - Meléndez-Martínez, Antonio Jesús

AU - Quintero-Gutiérrez, Adrián Guillermo

AU - Paredes-López, Octavio

AU - Del Villar-Martínez, Alma Angélica

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N2 - Marigold (Tagetes erecta) flowers are primarily used in industry for their high pigment content. Flower color development implies that chloroplast-chromoplast transition is associated with carotenoid biosynthesis. We report the recovery of undifferentiated pigmented marigold cells, various callus tissues, and their analysis by transmission electron microscopy in order to observe accumulating pigment and development of subcellular structures. Callus was generated from leaf explants and after several rounds of recurrent selection. Green-, yellow-, and brown-colored callus were obtained that showed distinct carotenoid profiles. For green material, violaxanthin, lutein, zeaxanthin, and β-carotene were produced, while yellow callus generated mainly lutein, as did the brown callus. Chloroplast-chromoplast transition was followed by measuring plastid size and shape in undifferentiated marigold cells by digital image analysis. Cellular alterations were evident in brown callus. Chloroplasts were the main structure in green callus, while yellow callus clearly showed the formation of plastoglobules, structures that are correlated with chloroplast-chromoplast transition. The high number of plastoglobules observed in yellow callus is possibly directly related to pigment synthesis and accumulation. © 2011 The Society for In Vitro Biology.

AB - Marigold (Tagetes erecta) flowers are primarily used in industry for their high pigment content. Flower color development implies that chloroplast-chromoplast transition is associated with carotenoid biosynthesis. We report the recovery of undifferentiated pigmented marigold cells, various callus tissues, and their analysis by transmission electron microscopy in order to observe accumulating pigment and development of subcellular structures. Callus was generated from leaf explants and after several rounds of recurrent selection. Green-, yellow-, and brown-colored callus were obtained that showed distinct carotenoid profiles. For green material, violaxanthin, lutein, zeaxanthin, and β-carotene were produced, while yellow callus generated mainly lutein, as did the brown callus. Chloroplast-chromoplast transition was followed by measuring plastid size and shape in undifferentiated marigold cells by digital image analysis. Cellular alterations were evident in brown callus. Chloroplasts were the main structure in green callus, while yellow callus clearly showed the formation of plastoglobules, structures that are correlated with chloroplast-chromoplast transition. The high number of plastoglobules observed in yellow callus is possibly directly related to pigment synthesis and accumulation. © 2011 The Society for In Vitro Biology.

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