TY - JOUR
T1 - Plant growth-promoting bacteria isolated from wild legume nodules and nodules of Phaseolus vulgaris L. trap plants in central and southern Mexico
AU - Tapia-García, Erika Yanet
AU - Hernández-Trejo, Verónica
AU - Guevara-Luna, Joseph
AU - Rojas-Rojas, Fernando Uriel
AU - Arroyo-Herrera, Ivan
AU - Meza-Radilla, Georgina
AU - Vásquez-Murrieta, María Soledad
AU - Estrada-de los Santos, Paulina
N1 - Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2020/10
Y1 - 2020/10
N2 - Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene sequence analysis, tested for plant growth-promoting (PGP) activities and the production of antimicrobial compounds, and analyzed for potential nodulation by amplifying the nodC gene. Several genera with PGP activity were isolated from legume nodules, including Achromobacter, Acinetobacter, Bacillus, Brevibacillus, Brevibacterium, Burkholderia, Cupriavidus, Dyella, Ensifer, Enterobacter, Herbaspirillum, Kosakonia, Labrys, Microbacterium, Moraxella, Paraburkholderia, Pseudomonas, Rhizobium, Stenotrophomonas; and Aeromonas, Marinococcus Pseudarthrobacter and Pseudoxanthomonas were found in plant legume nodules for the first time. Pseudomonas was the most common bacteria, and Mimosa pudica was colonized by the largest number of genera (6 different genera). A Burkholderia strain from the Burkholderia cepacia complex and a firmicutes strain harbor the nodC gene, identifying them as potential novel nodulating bacteria and showing that most of the strains isolated in this study were NAB. The most frequent PGP activity identified among the strains isolated from wild legumes was IAA synthesis. Two bacteria, Stenotrophomonas sp. and Rhizobium sp., synthesized more than 250 μg/ml, which is more than the level of synthesis reported in this study for Azospirillum brasilense Sp7 (59.77 μg/ml). Nitrogen fixation and antimicrobial compound production were not common, but the production of siderophores was frequently found among all the strains. This study shows that diverse NAB with PGP activity are very common in the legume nodules from central southern Mexico.
AB - Central southern Mexico contains highly diverse legumes. In this study, nodule-associated bacteria (NAB) were isolated from wild legume nodules and from nodules on Phaseolus vulgaris plants used as a plant-trap in soils from the same areas as the wild legumes. The bacteria were identified through the 16S rRNA gene sequence analysis, tested for plant growth-promoting (PGP) activities and the production of antimicrobial compounds, and analyzed for potential nodulation by amplifying the nodC gene. Several genera with PGP activity were isolated from legume nodules, including Achromobacter, Acinetobacter, Bacillus, Brevibacillus, Brevibacterium, Burkholderia, Cupriavidus, Dyella, Ensifer, Enterobacter, Herbaspirillum, Kosakonia, Labrys, Microbacterium, Moraxella, Paraburkholderia, Pseudomonas, Rhizobium, Stenotrophomonas; and Aeromonas, Marinococcus Pseudarthrobacter and Pseudoxanthomonas were found in plant legume nodules for the first time. Pseudomonas was the most common bacteria, and Mimosa pudica was colonized by the largest number of genera (6 different genera). A Burkholderia strain from the Burkholderia cepacia complex and a firmicutes strain harbor the nodC gene, identifying them as potential novel nodulating bacteria and showing that most of the strains isolated in this study were NAB. The most frequent PGP activity identified among the strains isolated from wild legumes was IAA synthesis. Two bacteria, Stenotrophomonas sp. and Rhizobium sp., synthesized more than 250 μg/ml, which is more than the level of synthesis reported in this study for Azospirillum brasilense Sp7 (59.77 μg/ml). Nitrogen fixation and antimicrobial compound production were not common, but the production of siderophores was frequently found among all the strains. This study shows that diverse NAB with PGP activity are very common in the legume nodules from central southern Mexico.
KW - Biological nitrogen fixation
KW - Mimosa
KW - Nodulation
KW - Nodule associated bacteria
KW - Rhizosphere
UR - http://www.scopus.com/inward/record.url?scp=85086803103&partnerID=8YFLogxK
U2 - 10.1016/j.micres.2020.126522
DO - 10.1016/j.micres.2020.126522
M3 - Artículo
C2 - 32585580
AN - SCOPUS:85086803103
SN - 0944-5013
VL - 239
JO - Microbiological Research
JF - Microbiological Research
M1 - 126522
ER -