TY - JOUR
T1 - Phylogenetic position of geosmithia spp. (hypocreales) living in juniperus spp. forests (cupressaceae) with bark beetles of phloeosinus spp. (scolytinae) from the northeast of mexico
AU - Alfredo, Hernández García Juan
AU - Gerardo, Cuellar Rodríguez
AU - Guadalupe, Aguirre Ojeda Nallely
AU - Lourdes, Villa Tanaca
AU - Cesar, Hernández Rodríguez
AU - Francisco, Armendáriz Toledano
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/11
Y1 - 2020/11
N2 - Geosmithia members are mitosporic filamentous fungi commonly recorded and isolated from bark beetles of the Scolytinae subfamily and their respective host’s species. This genus includes 18 species formally described and 38 phylogenetic species recorded in several localities from Africa, Asia, Australia, Europe, and North and South America, where they exhibit frequent associations with phloeophagous and wood-boring bark beetles. Among phloephagous bark beetle species, specifically, in members of the genus Phloeosinus Chapuis, almost 10% of Geosmithia strains have been isolated. By its physiographic elements and high bark beetle and conifer species richness, Mexico is a potential region to host a high diversity of Geosmithia species and potential new species. In the present study, we systematically sampled and isolated, cultured, and molecularly identified members of the Geosmithia species associated with Phloeosinus spp. and their Juniperus spp. host trees at the north of Sierra Madre Oriental, at Nuevo Leon State, Mexico. Phylogenetic analyses based on 378 internal transcribed spacer region (ITS) sequences supported the presence of strains from Geosmithia langdonii-Geosmithia sp. 32 clade associated with Phloeosinus serratus vector and with Juniperus coahuilensis (JC) host, and the presence of strains from Geosmithia sp. 21-Geosmithia xerotolerans clade with Phloeosinus deleoni and Juniperus flaccida (JF)in this geographical region. The genetic and morphological differences found in our strains with respect to those previously described in the species from both clades (Geosmithia langdonii-Geosmithia sp. 32 and Geosmithia sp. 21-G. xerotolerans) suggest that both Geosmithia lineages from Nuevo Leon correspond to two potential new species in the genus.
AB - Geosmithia members are mitosporic filamentous fungi commonly recorded and isolated from bark beetles of the Scolytinae subfamily and their respective host’s species. This genus includes 18 species formally described and 38 phylogenetic species recorded in several localities from Africa, Asia, Australia, Europe, and North and South America, where they exhibit frequent associations with phloeophagous and wood-boring bark beetles. Among phloephagous bark beetle species, specifically, in members of the genus Phloeosinus Chapuis, almost 10% of Geosmithia strains have been isolated. By its physiographic elements and high bark beetle and conifer species richness, Mexico is a potential region to host a high diversity of Geosmithia species and potential new species. In the present study, we systematically sampled and isolated, cultured, and molecularly identified members of the Geosmithia species associated with Phloeosinus spp. and their Juniperus spp. host trees at the north of Sierra Madre Oriental, at Nuevo Leon State, Mexico. Phylogenetic analyses based on 378 internal transcribed spacer region (ITS) sequences supported the presence of strains from Geosmithia langdonii-Geosmithia sp. 32 clade associated with Phloeosinus serratus vector and with Juniperus coahuilensis (JC) host, and the presence of strains from Geosmithia sp. 21-Geosmithia xerotolerans clade with Phloeosinus deleoni and Juniperus flaccida (JF)in this geographical region. The genetic and morphological differences found in our strains with respect to those previously described in the species from both clades (Geosmithia langdonii-Geosmithia sp. 32 and Geosmithia sp. 21-G. xerotolerans) suggest that both Geosmithia lineages from Nuevo Leon correspond to two potential new species in the genus.
KW - Bark beetles
KW - Geosmithia
KW - Juniperus
KW - Phloeosinus
UR - http://www.scopus.com/inward/record.url?scp=85094676168&partnerID=8YFLogxK
U2 - 10.3390/f11111142
DO - 10.3390/f11111142
M3 - Artículo
AN - SCOPUS:85094676168
SN - 1999-4907
VL - 11
SP - 1
EP - 19
JO - Forests
JF - Forests
IS - 11
M1 - 1142
ER -