TY - JOUR
T1 - Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia
AU - Tan, Zhi Yuan
AU - Xu, Xiao Dong
AU - Wang, E. N.Tao
AU - Gao, Jun Lian
AU - Martinez-Romero, Esperanza
AU - Chen, Wen Xin
PY - 1997/7
Y1 - 1997/7
N2 - The genetic and phylogenetic relationships for strains of Mesorhizobium tianshanense and its relatives were compared by an analysis of the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins, DNA-DNA hybridization, and full 16S rRNA gene sequencing. The strains of M. tianshanense formed a cluster which was distinct from those of other rhizobium species in the clustering analysis of SDS-PAGE. DNA-DNA relatedness between A-1BS (type strain of M. tianshanense) and the type or reference strains for Mesorhizobium loti, M. huakuii, M. ciceri, M. mediterraneum, and cluster U, an unnamed rhizobial group, ranged from 4.4 to 43.8%. The phylogenetic analysis based on the 16S rRNA gene sequences showed that M. tianshanense was closely related to the Mesorhizobium phylogenetic branch and could be distinguished from the other four species in this branch. These results further confirmed that these bacteria constitute a distinct rhizobial species.
AB - The genetic and phylogenetic relationships for strains of Mesorhizobium tianshanense and its relatives were compared by an analysis of the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins, DNA-DNA hybridization, and full 16S rRNA gene sequencing. The strains of M. tianshanense formed a cluster which was distinct from those of other rhizobium species in the clustering analysis of SDS-PAGE. DNA-DNA relatedness between A-1BS (type strain of M. tianshanense) and the type or reference strains for Mesorhizobium loti, M. huakuii, M. ciceri, M. mediterraneum, and cluster U, an unnamed rhizobial group, ranged from 4.4 to 43.8%. The phylogenetic analysis based on the 16S rRNA gene sequences showed that M. tianshanense was closely related to the Mesorhizobium phylogenetic branch and could be distinguished from the other four species in this branch. These results further confirmed that these bacteria constitute a distinct rhizobial species.
UR - http://www.scopus.com/inward/record.url?scp=0030876644&partnerID=8YFLogxK
U2 - 10.1099/00207713-47-3-874
DO - 10.1099/00207713-47-3-874
M3 - Artículo
C2 - 9226921
AN - SCOPUS:0030876644
SN - 0020-7713
VL - 47
SP - 874
EP - 879
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
IS - 3
ER -