TY - JOUR
T1 - Photosynthesis and photoprotection characteristics related to ROS production in three Chattonella (Raphidophyceae) species
AU - Ahumada-Fierro, Norma Valeria
AU - García-Mendoza, Ernesto
AU - Sandoval-Gil, Jose Miguel
AU - Band-Schmidt, Christine Johanna
N1 - Publisher Copyright:
© 2021 Phycological Society of America
PY - 2021/6
Y1 - 2021/6
N2 - The toxic potential of Chattonella is associated with a high production of reactive oxygen species (ROS). Chattonella species can tolerate high irradiance levels but seems not to be efficient in the induction of nonphotochemical chl a fluorescence (NPQ) under light stress conditions. Therefore, we postulated that high ROS production of this microalgal group is related to the lack of effective photoprotection mechanisms. We compared the NPQ induction, xanthophyll cycle interconversion (XC), and the production of the ion superoxide (O2−) in Chattonella marina var. antiqua, Chattonella sp., and C. marina acclimated to 43 (LL) and 300 µmol photons · m2 · s−1 (HL). We also evaluated the photosynthetic characteristics of the three strains. Photosynthesis saturated at relative high irradiances (above 500 µmol photons · m2 · s−1) in LL and HL Chattonella strains. For the first time, we documented the conversion of diadinoxanthin into diatoxanthin in microalgae that have violaxanthin as the major XC carotenoid. The slow NPQ induction indicated that qE (fast component of NPQ) was not present, and this process was related to the interconversion of XC pigments. However, the quenching efficiency (QE) of deepoxidated xanthophylls was low in the three Chattonella strains. The strain with the lowest QE produced the highest amount of a O2−. Therefore, ROS production in Chattonella seems to be related to a low expression of XC-related thermal PSII dissipation.
AB - The toxic potential of Chattonella is associated with a high production of reactive oxygen species (ROS). Chattonella species can tolerate high irradiance levels but seems not to be efficient in the induction of nonphotochemical chl a fluorescence (NPQ) under light stress conditions. Therefore, we postulated that high ROS production of this microalgal group is related to the lack of effective photoprotection mechanisms. We compared the NPQ induction, xanthophyll cycle interconversion (XC), and the production of the ion superoxide (O2−) in Chattonella marina var. antiqua, Chattonella sp., and C. marina acclimated to 43 (LL) and 300 µmol photons · m2 · s−1 (HL). We also evaluated the photosynthetic characteristics of the three strains. Photosynthesis saturated at relative high irradiances (above 500 µmol photons · m2 · s−1) in LL and HL Chattonella strains. For the first time, we documented the conversion of diadinoxanthin into diatoxanthin in microalgae that have violaxanthin as the major XC carotenoid. The slow NPQ induction indicated that qE (fast component of NPQ) was not present, and this process was related to the interconversion of XC pigments. However, the quenching efficiency (QE) of deepoxidated xanthophylls was low in the three Chattonella strains. The strain with the lowest QE produced the highest amount of a O2−. Therefore, ROS production in Chattonella seems to be related to a low expression of XC-related thermal PSII dissipation.
KW - diatoxanthin
KW - nonphotochemical quenching
KW - photoprotection
KW - photosynthesis
KW - raphidophytes
KW - xanthophyll cycle
KW - zeaxanthin
UR - http://www.scopus.com/inward/record.url?scp=85103164561&partnerID=8YFLogxK
U2 - 10.1111/jpy.13138
DO - 10.1111/jpy.13138
M3 - Artículo
C2 - 33523492
AN - SCOPUS:85103164561
SN - 0022-3646
VL - 57
SP - 941
EP - 954
JO - Journal of Phycology
JF - Journal of Phycology
IS - 3
ER -