TY - JOUR
T1 - Phenylpropanoids are connected to cell wall fortification and stress tolerance in avocado somatic embryogenesis
AU - Olivares-García, Carol A.
AU - Mata-Rosas, Martín
AU - Peña-Montes, Carolina
AU - Quiroz-Figueroa, Francisco
AU - Segura-Cabrera, Aldo
AU - Shannon, Laura M.
AU - Loyola-Vargas, Victor M.
AU - Monribot-Villanueva, Juan L.
AU - Elizalde-Contreras, Jose M.
AU - Ibarra-Laclette, Enrique
AU - Ramirez-Vázquez, Mónica
AU - Guerrero-Analco, José A.
AU - Ruiz-May, Eliel
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/8/2
Y1 - 2020/8/2
N2 - Somatic embryogenesis (SE) is a valuable model for understanding the mechanism of plant embryogenesis and a tool for the mass production of plants. However, establishing SE in avocado has been complicated due to the very low efficiency of embryo induction and plant regeneration. To understand the molecular foundation of the SE induction and development in avocado, we compared embryogenic (EC) and non-embryogenic (NEC) cultures of two avocado varieties using proteomic and metabolomic approaches. Although Criollo and Hass EC exhibited similarities in the proteome and metabolome profile, in general, we observed a more active phenylpropanoid pathway in EC than NEC. This pathway is associated with the tolerance of stress responses, probably through the reinforcement of the cell wall and flavonoid production. We could corroborate that particular polyphenolics compounds, including p-coumaric acid and t-ferulic acid, stimulated the production of somatic embryos in avocado. Exogen phenolic compounds were associated with the modification of the content of endogenous polyphenolic and the induction of the production of the putative auxin-a, adenosine, cellulose and 1,26-hexacosanediol-diferulate. We suggest that in EC of avocado, there is an enhanced phenylpropanoid metabolism for the production of the building blocks of lignin and flavonoid compounds having a role in cell wall reinforcement for tolerating stress response. Data are available at ProteomeXchange with the identifier PXD019705.
AB - Somatic embryogenesis (SE) is a valuable model for understanding the mechanism of plant embryogenesis and a tool for the mass production of plants. However, establishing SE in avocado has been complicated due to the very low efficiency of embryo induction and plant regeneration. To understand the molecular foundation of the SE induction and development in avocado, we compared embryogenic (EC) and non-embryogenic (NEC) cultures of two avocado varieties using proteomic and metabolomic approaches. Although Criollo and Hass EC exhibited similarities in the proteome and metabolome profile, in general, we observed a more active phenylpropanoid pathway in EC than NEC. This pathway is associated with the tolerance of stress responses, probably through the reinforcement of the cell wall and flavonoid production. We could corroborate that particular polyphenolics compounds, including p-coumaric acid and t-ferulic acid, stimulated the production of somatic embryos in avocado. Exogen phenolic compounds were associated with the modification of the content of endogenous polyphenolic and the induction of the production of the putative auxin-a, adenosine, cellulose and 1,26-hexacosanediol-diferulate. We suggest that in EC of avocado, there is an enhanced phenylpropanoid metabolism for the production of the building blocks of lignin and flavonoid compounds having a role in cell wall reinforcement for tolerating stress response. Data are available at ProteomeXchange with the identifier PXD019705.
KW - Embryogenic cultures
KW - Metabolomics
KW - Phenolic compounds
KW - Plant cell wall
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=85089408207&partnerID=8YFLogxK
U2 - 10.3390/ijms21165679
DO - 10.3390/ijms21165679
M3 - Artículo
C2 - 32784357
AN - SCOPUS:85089408207
SN - 1661-6596
VL - 21
SP - 1
EP - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 16
M1 - 5679
ER -