TY - JOUR
T1 - Phenylpropanoid production in callus and cell suspension cultures of Buddleja cordata Kunth
AU - Estrada-Zúñiga, M. E.
AU - Cruz-Sosa, F.
AU - Rodríguez-Monroy, M.
AU - Verde-Calvo, J. R.
AU - Vernon-Carter, E. J.
N1 - Funding Information:
Acknowledgements The authors thank the Programa de Apoyo de la Investigación Multidisciplinario (Desarrollo de fitomedicamentos a partir de plantas medicinales y sus extractos para el control de la diabetes mellitus) for the partial financing of this project, and the Consejo Nacional de Ciencia y Tecnología (CONACyT) through grant 81157. We also thank Biologist Jorge Santana Carrillo at UAM-I herbarium for taxonomic identification of the collected plant as Buddleja cordata.
PY - 2009/4
Y1 - 2009/4
N2 - Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata tissue cultures were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids), as these metabolites are credited with therapeutic properties. Highest callus induction (76.4-84.3%) was obtained in five treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-d: 0.45-9.05 μM) with Kinetin (KIN: 2.32, 4.65 μM), whereas highest root induction (79.6%) corresponded to the α-Naphthaleneacetic acid (9.05 μM) with KIN (2.32 μM) treatment. Verbascoside was the major phenylpropanoid produced in in vitro cultures (root, white and green callus) [66.24-86.26 mg g-1 dry weight (DW)], while linarin and hydroxycinnamic acid production was low (0.95-3.01 mg g-1 DW). Verbascoside and linarin production were improved in cell suspension culture (116 mg g-1 DW and 8.12 mg g-1 DW, respectively).
AB - Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata tissue cultures were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids), as these metabolites are credited with therapeutic properties. Highest callus induction (76.4-84.3%) was obtained in five treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-d: 0.45-9.05 μM) with Kinetin (KIN: 2.32, 4.65 μM), whereas highest root induction (79.6%) corresponded to the α-Naphthaleneacetic acid (9.05 μM) with KIN (2.32 μM) treatment. Verbascoside was the major phenylpropanoid produced in in vitro cultures (root, white and green callus) [66.24-86.26 mg g-1 dry weight (DW)], while linarin and hydroxycinnamic acid production was low (0.95-3.01 mg g-1 DW). Verbascoside and linarin production were improved in cell suspension culture (116 mg g-1 DW and 8.12 mg g-1 DW, respectively).
KW - Buddleja cordata
KW - Hydroxycinnamic acids
KW - Linarin
KW - Secondary metabolite
KW - Tissue culture
KW - Verbascoside
UR - http://www.scopus.com/inward/record.url?scp=61449246355&partnerID=8YFLogxK
U2 - 10.1007/s11240-009-9496-z
DO - 10.1007/s11240-009-9496-z
M3 - Artículo
SN - 0167-6857
VL - 97
SP - 39
EP - 47
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
IS - 1
ER -