TY - JOUR
T1 - Phenotypic characterization of a trifluoperazine-resistant mutant ofMucor rouxii
AU - Vazquez-Marrufo, Gerardo
AU - Castillo-Ramirez, Eduardo
AU - Salgado-Rodriguez, L. Miguel
AU - Gutierrez-Corona, J. Felix
AU - Martinez-Cadena, Guadalupe
N1 - Funding Information:
We thank Drs. E. Lopez-Romero and I. Garcia-Soto for critical reading of the manuscript. Part of this work was supported by DIGICySA of the Subsecretaria de Education Superior e Investigation Cientifica, SEP; and the Consejo National de Ciencia y Tecnologia, Mexico.
PY - 1992/6
Y1 - 1992/6
N2 - A trifluoperazine-resistant (TFP1) mutant (strain G5) ofMucor rouxii was isolated and some biochemical and physiological parameters were studied. It resisted up to 250 μM TFP compared to 100 μM observed for the wild-type strain. At this drug concentration the mutant strain G5 germinated, grew, exhibited yeast-mycelium transition, and chitin synthesisin vivo. The mutant strain presentedin vitro levels of calmodulin activity similar to those of the wild-type, but with less sensitivity to inhibition by TFP. Also, with regard to spore germination and cell growth, mutant G5 presented cross-resistance to calmidazolium, another potent anticalmodulin drug. Partially purified chitin synthetase preparations of mutant G5 exhibited a diminished enzymatic activity, compared to the wild-type. The results presented in this work suggest the participation of a Ca2+-calmodulin complex in growth and differentiative processes ofMucor and substantiate the role of this complex in chitin synthesis.
AB - A trifluoperazine-resistant (TFP1) mutant (strain G5) ofMucor rouxii was isolated and some biochemical and physiological parameters were studied. It resisted up to 250 μM TFP compared to 100 μM observed for the wild-type strain. At this drug concentration the mutant strain G5 germinated, grew, exhibited yeast-mycelium transition, and chitin synthesisin vivo. The mutant strain presentedin vitro levels of calmodulin activity similar to those of the wild-type, but with less sensitivity to inhibition by TFP. Also, with regard to spore germination and cell growth, mutant G5 presented cross-resistance to calmidazolium, another potent anticalmodulin drug. Partially purified chitin synthetase preparations of mutant G5 exhibited a diminished enzymatic activity, compared to the wild-type. The results presented in this work suggest the participation of a Ca2+-calmodulin complex in growth and differentiative processes ofMucor and substantiate the role of this complex in chitin synthesis.
KW - Mucor rouxii
KW - calmidazolium
KW - calmodulin
KW - chitin synthesis
KW - differentiation
KW - mutants
KW - trifluoperazine
UR - http://www.scopus.com/inward/record.url?scp=9444276120&partnerID=8YFLogxK
U2 - 10.1016/0147-5975(92)90021-I
DO - 10.1016/0147-5975(92)90021-I
M3 - Artículo
AN - SCOPUS:9444276120
SN - 0147-5975
VL - 16
SP - 146
EP - 154
JO - Experimental Mycology
JF - Experimental Mycology
IS - 2
ER -