Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance

Ofelia E. Carreón-Rodríguez; Rosa María Gutiérrez-Ríos; José L. Acosta; Alfredo Martinez; Miguel A. Cevallos

doi:10.1016/j.btre.2019.e00328

Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance

Ofelia E. Carreón-Rodríguez, Rosa María Gutiérrez-Ríos, José L. Acosta, Alfredo Martinez, Miguel A. Cevallos

Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional (CIIDIR), Unidad Sinaloa

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Zymomonas mobilis ZM4 is an ethanol-producing microbe that is constitutively tolerant to this solvent. For a better understanding of the ethanol tolerance phenomenon we obtained and characterized two ZM4 mutants (ER79ap and ER79ag) with higher ethanol tolerance than the wild-type. Mutants were evaluated in different ethanol concentrations and this analysis showed that mutant ER79ap was more tolerant and had a better performance in terms of cell viability, than the wild-type strain and ER79ag mutant. Genotyping of the mutant strains showed that both carry non-synonymous mutations in clpP and spoT/relA genes. A third non-synonymous mutation was found only in strain ER79ap, in the clpB gene. Considering that ER79ap has the best tolerance to added ethanol, the mutant alleles of this strain were evaluated in ZM4 and here we show that while all of them contribute to ethanol tolerance, mutation within spoT/relA gene seems to be the most important.

Original language	English
Article number	e00328
Journal	Biotechnology Reports
Volume	23
DOIs	https://doi.org/10.1016/j.btre.2019.e00328
State	Published - Sep 2019

Keywords

Adaptive evolution
Ethanol tolerance
Genome sequencing
Inoculum size
Zymomonas mobilis
spoT/relA

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10.1016/j.btre.2019.e00328

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title = "Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance",

abstract = "Zymomonas mobilis ZM4 is an ethanol-producing microbe that is constitutively tolerant to this solvent. For a better understanding of the ethanol tolerance phenomenon we obtained and characterized two ZM4 mutants (ER79ap and ER79ag) with higher ethanol tolerance than the wild-type. Mutants were evaluated in different ethanol concentrations and this analysis showed that mutant ER79ap was more tolerant and had a better performance in terms of cell viability, than the wild-type strain and ER79ag mutant. Genotyping of the mutant strains showed that both carry non-synonymous mutations in clpP and spoT/relA genes. A third non-synonymous mutation was found only in strain ER79ap, in the clpB gene. Considering that ER79ap has the best tolerance to added ethanol, the mutant alleles of this strain were evaluated in ZM4 and here we show that while all of them contribute to ethanol tolerance, mutation within spoT/relA gene seems to be the most important.",

keywords = "Adaptive evolution, Ethanol tolerance, Genome sequencing, Inoculum size, Zymomonas mobilis, spoT/relA",

author = "Carre{\'o}n-Rodr{\'i}guez, {Ofelia E.} and Guti{\'e}rrez-R{\'i}os, {Rosa Mar{\'i}a} and Acosta, {Jos{\'e} L.} and Alfredo Martinez and Cevallos, {Miguel A.}",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = sep,

doi = "10.1016/j.btre.2019.e00328",

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T1 - Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance

AU - Carreón-Rodríguez, Ofelia E.

AU - Gutiérrez-Ríos, Rosa María

AU - Acosta, José L.

AU - Martinez, Alfredo

AU - Cevallos, Miguel A.

PY - 2019/9

Y1 - 2019/9

N2 - Zymomonas mobilis ZM4 is an ethanol-producing microbe that is constitutively tolerant to this solvent. For a better understanding of the ethanol tolerance phenomenon we obtained and characterized two ZM4 mutants (ER79ap and ER79ag) with higher ethanol tolerance than the wild-type. Mutants were evaluated in different ethanol concentrations and this analysis showed that mutant ER79ap was more tolerant and had a better performance in terms of cell viability, than the wild-type strain and ER79ag mutant. Genotyping of the mutant strains showed that both carry non-synonymous mutations in clpP and spoT/relA genes. A third non-synonymous mutation was found only in strain ER79ap, in the clpB gene. Considering that ER79ap has the best tolerance to added ethanol, the mutant alleles of this strain were evaluated in ZM4 and here we show that while all of them contribute to ethanol tolerance, mutation within spoT/relA gene seems to be the most important.

AB - Zymomonas mobilis ZM4 is an ethanol-producing microbe that is constitutively tolerant to this solvent. For a better understanding of the ethanol tolerance phenomenon we obtained and characterized two ZM4 mutants (ER79ap and ER79ag) with higher ethanol tolerance than the wild-type. Mutants were evaluated in different ethanol concentrations and this analysis showed that mutant ER79ap was more tolerant and had a better performance in terms of cell viability, than the wild-type strain and ER79ag mutant. Genotyping of the mutant strains showed that both carry non-synonymous mutations in clpP and spoT/relA genes. A third non-synonymous mutation was found only in strain ER79ap, in the clpB gene. Considering that ER79ap has the best tolerance to added ethanol, the mutant alleles of this strain were evaluated in ZM4 and here we show that while all of them contribute to ethanol tolerance, mutation within spoT/relA gene seems to be the most important.

KW - Adaptive evolution

KW - Ethanol tolerance

KW - Genome sequencing

KW - Inoculum size

KW - Zymomonas mobilis

KW - spoT/relA

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DO - 10.1016/j.btre.2019.e00328

M3 - Artículo

AN - SCOPUS:85063337687

SN - 2215-017X

VL - 23

JO - Biotechnology Reports

JF - Biotechnology Reports

M1 - e00328

ER -

Phenotypic and genomic analysis of Zymomonas mobilis ZM4 mutants with enhanced ethanol tolerance

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Keywords

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