TY - JOUR
T1 - Phenolic compounds inhibit cellulase and xylanase activities of Cellulomonas flavigena PR-22 during saccharification of sugarcane bagasse
AU - González-Bautista, Enrique
AU - Santana-Morales, Juan Carlos
AU - Ríos-Fránquez, Francisco Javier
AU - Poggi-Varaldo, Héctor Mario
AU - Ramos-Valdivia, Ana Carmela
AU - Cristiani-Urbina, Eliseo
AU - Ponce-Noyola, Teresa
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017
Y1 - 2017
N2 - The enzymatic hydrolysis of sugar cane bagasse (SCB) releases simple fermentable sugars however, phenolic compounds (PCs) are also released from the lignin present in the structure of the lignocellulosic biomass limiting the production of sugars and therefore the fermentative process to convert these sugars to bioethanol. During the saccharification of SCB with holocellulases from Cellulomonas flavigena PR-22, 46 μg mL−1of PCs were produced, which inhibited 87 and 74% carboxymethyl cellulase (CMCase) and xylanases respectively resulting in a low concentration of reducing sugars (5.5 g L−1) at the end of the process. Polymers such as polyethylene glycol 4000 (PEG) and polyvinylpyrrolidone (PVP) can prevent this inhibition due to their ability to chemically adsorb PCs. PEG and PVP were added to C. flavigena PR-22 enzyme extracts at 0.4% (w/v). PEG 4000 prevented CMCase inhibition, maintaining the original activity after the saccharification process, whereas xylanase production was increased by 47% when PVP was added.
AB - The enzymatic hydrolysis of sugar cane bagasse (SCB) releases simple fermentable sugars however, phenolic compounds (PCs) are also released from the lignin present in the structure of the lignocellulosic biomass limiting the production of sugars and therefore the fermentative process to convert these sugars to bioethanol. During the saccharification of SCB with holocellulases from Cellulomonas flavigena PR-22, 46 μg mL−1of PCs were produced, which inhibited 87 and 74% carboxymethyl cellulase (CMCase) and xylanases respectively resulting in a low concentration of reducing sugars (5.5 g L−1) at the end of the process. Polymers such as polyethylene glycol 4000 (PEG) and polyvinylpyrrolidone (PVP) can prevent this inhibition due to their ability to chemically adsorb PCs. PEG and PVP were added to C. flavigena PR-22 enzyme extracts at 0.4% (w/v). PEG 4000 prevented CMCase inhibition, maintaining the original activity after the saccharification process, whereas xylanase production was increased by 47% when PVP was added.
KW - Cellulomonas flavigena
KW - Holocellulases
KW - Phenolic compounds
KW - Saccharification
UR - http://www.scopus.com/inward/record.url?scp=85011333651&partnerID=8YFLogxK
U2 - 10.1016/j.fuel.2017.01.080
DO - 10.1016/j.fuel.2017.01.080
M3 - Artículo
SN - 0016-2361
VL - 196
SP - 32
EP - 35
JO - Fuel
JF - Fuel
ER -