TY - JOUR
T1 - Peroxidase-mediated transformation of hydroxy-9,10-anthraquinones
AU - Arrieta-Baez, Daniel
AU - Roman, Rosa
AU - Vazquez-Duhalt, Rafael
AU - Jiménez-Estrada, Manuel
N1 - Funding Information:
This work was funded by CONACyT (No. 91988), PAEP (103 302) and PAPIIT (IN206999). Contribution 1754 IQUANAM.
PY - 2002
Y1 - 2002
N2 - A peroxidase (EC 1.11.1.7) has been isolated and purified from Senna angustifolia. The enzyme was purified by ion-exchange chromatography on high Q and high S columns. SDS-PAGE electrophoresis showed that the protein has a molecular mass of approximately 70 kDa. Hydroxy-anthraquinones and hydroxy-anthracenones were evaluated as substrate of S. angustifolia and horseradish peroxidases. Both peroxidases catalyzed the oxidation of alizarin and purpurin anthraquinones to the corresponding 3,3′-bializarin and the new compound 3,3′-bipurpurin, respectively, as well as the formation of 2,2′-biquinizarin from quinizarin anthracenone. The KMapp and Vmax values for alizarin and purpurin were 97 and 95 μM and 1.5 and 2.1 μM min-1 mg prot-1, respectively. The results suggest that peroxidase may participate in the biogenesis of anthraquinones.
AB - A peroxidase (EC 1.11.1.7) has been isolated and purified from Senna angustifolia. The enzyme was purified by ion-exchange chromatography on high Q and high S columns. SDS-PAGE electrophoresis showed that the protein has a molecular mass of approximately 70 kDa. Hydroxy-anthraquinones and hydroxy-anthracenones were evaluated as substrate of S. angustifolia and horseradish peroxidases. Both peroxidases catalyzed the oxidation of alizarin and purpurin anthraquinones to the corresponding 3,3′-bializarin and the new compound 3,3′-bipurpurin, respectively, as well as the formation of 2,2′-biquinizarin from quinizarin anthracenone. The KMapp and Vmax values for alizarin and purpurin were 97 and 95 μM and 1.5 and 2.1 μM min-1 mg prot-1, respectively. The results suggest that peroxidase may participate in the biogenesis of anthraquinones.
KW - Anthracenones
KW - Bianthraquinones
KW - Horseradish peroxidase
KW - Kinetic properties
KW - Leguminosae
KW - Phenolic oxidation
KW - Senna angustifolia
UR - http://www.scopus.com/inward/record.url?scp=0035983822&partnerID=8YFLogxK
U2 - 10.1016/S0031-9422(02)00173-5
DO - 10.1016/S0031-9422(02)00173-5
M3 - Artículo
SN - 0031-9422
VL - 60
SP - 567
EP - 572
JO - Phytochemistry
JF - Phytochemistry
IS - 6
ER -