TY - JOUR
T1 - Pathogenic CCR6+ dendritic cells in the skin lesions of discoid lupus patients
T2 - A role for damage-associated molecular patterns
AU - Méndez-Reguera, Aniela
AU - Pérez-Montesinos, Gibrán
AU - Ntarahernández, Marcela Alcá
AU - Mart́inez-Estrada, Virginia
AU - Cazarinbarrientos, Jorge Rafael
AU - Rojas-Espinosa, Oscar
AU - Jurado-Santacruz, Fermín
AU - Huerta-Yepez, Sara
AU - Bonifaz-Alfonzo, Laura
PY - 2013/3
Y1 - 2013/3
N2 - Background: Discoid lupus erythematosus (DLE) is a cutaneous autoimmune inflammatory disease in which the role of conventional dendritic cells (cDCs) in skin damage has not been evaluated. Objective:To evaluate the involvement of cDCs inDLEpathogenesis. Material & Methods: Skin biopsies from 42 patients with DLE were embedded in paraffin or placed in culture. The dermis was separated and cell suspensions were characterized by flow cytometry. Results: We found an increase in cDCs with inflammatory characteristics in the skin of DLE patients, compared with control skins. Interestingly, cDCs from the DLE patients expressed low levels of the inhibitory molecule PD-L1 and showed a high expression of CCR6, which correlated with disease activity. Increased cellular death was observed in the skin of DLE patients compared with control skin and remarkably we found that damage-associated molecular patterns could be responsible for CCR6 expression on cDCs in the skin. Conclusions: Our results indicate the presence of pathogenic CCR6+ cDCs in the skin lesions of DLE patients, which could result from in situ phenotypic changes.
AB - Background: Discoid lupus erythematosus (DLE) is a cutaneous autoimmune inflammatory disease in which the role of conventional dendritic cells (cDCs) in skin damage has not been evaluated. Objective:To evaluate the involvement of cDCs inDLEpathogenesis. Material & Methods: Skin biopsies from 42 patients with DLE were embedded in paraffin or placed in culture. The dermis was separated and cell suspensions were characterized by flow cytometry. Results: We found an increase in cDCs with inflammatory characteristics in the skin of DLE patients, compared with control skins. Interestingly, cDCs from the DLE patients expressed low levels of the inhibitory molecule PD-L1 and showed a high expression of CCR6, which correlated with disease activity. Increased cellular death was observed in the skin of DLE patients compared with control skin and remarkably we found that damage-associated molecular patterns could be responsible for CCR6 expression on cDCs in the skin. Conclusions: Our results indicate the presence of pathogenic CCR6+ cDCs in the skin lesions of DLE patients, which could result from in situ phenotypic changes.
KW - Damage associated molecular patterns
KW - Dendritic cells
KW - Dermal dendritic cells
KW - Discoid lupus erythematosus
KW - Inflammatory dendritic cells
UR - http://www.scopus.com/inward/record.url?scp=84877764602&partnerID=8YFLogxK
U2 - 10.1684/ejd.2013.1970
DO - 10.1684/ejd.2013.1970
M3 - Artículo
SN - 1167-1122
VL - 23
SP - 169
EP - 182
JO - European Journal of Dermatology
JF - European Journal of Dermatology
IS - 2
ER -