TY - JOUR
T1 - Oxidative stress indicators and trace element concentrations in tissues of mako shark (Isurus oxyrinchus)
AU - Vélez-Alavez, Marcela
AU - Labrada-Martagón, Vanessa
AU - Méndez-Rodriguez, Lía C.
AU - Galván-Magaña, Felipe
AU - Zenteno-Savín, Tania
N1 - Funding Information:
The authors acknowledge the fishing community of San Lazaro for samples obtained, and the support of O. Lugo-Lugo, N.O. Olguín-Monroy, B. Acosta-Vargas and G. Peña-Armenta who provided guidance and technical assistance in processing samples. Comments by two anonymous reviewers helped improve the article. This project was financed by CICIMAR (IPN) and CIBNOR ( PC2.0 , PC0.10 ). M.V.A. is a recipient of a scholarship from CONACYT ( 12949 ).
PY - 2013/8
Y1 - 2013/8
N2 - Liver, kidney and muscle from juvenile mako sharks (Isurus oxyrinchus) were collected in Baja California Sur. Lead (Pb), cadmium (Cd), mercury (Hg) and arsenic (As) concentrations were determined by atomic absorption spectrophotometry. The production of superoxide radical (O2•-) was measured as an indicator of reactive oxygen species production; lipid peroxidation (TBARS) and protein carbonyl levels were quantified as indicators of oxidative damage, and the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) was assessed as indicator of antioxidant defenses. Two discriminant functions separated muscle from liver and kidney samples. Cd concentration was lower in muscle than in kidney (p<0.05) and Hg concentration was higher in muscle than in liver and kidney (p<0.05). Although GR and SOD activities were higher, oxidative damage (TBARS and carbonyl protein levels) was also higher in kidney (p<0.05). SOD activity, TBARS levels, and Cd and Hg concentration were the set of predictors with significant relevance during tissue discrimination. Tissue metabolism, physiology of the organisms and environmental factors may be related to the differences in trace elements and oxidative stress indicators found in muscle, liver and kidney of the mako shark.
AB - Liver, kidney and muscle from juvenile mako sharks (Isurus oxyrinchus) were collected in Baja California Sur. Lead (Pb), cadmium (Cd), mercury (Hg) and arsenic (As) concentrations were determined by atomic absorption spectrophotometry. The production of superoxide radical (O2•-) was measured as an indicator of reactive oxygen species production; lipid peroxidation (TBARS) and protein carbonyl levels were quantified as indicators of oxidative damage, and the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) was assessed as indicator of antioxidant defenses. Two discriminant functions separated muscle from liver and kidney samples. Cd concentration was lower in muscle than in kidney (p<0.05) and Hg concentration was higher in muscle than in liver and kidney (p<0.05). Although GR and SOD activities were higher, oxidative damage (TBARS and carbonyl protein levels) was also higher in kidney (p<0.05). SOD activity, TBARS levels, and Cd and Hg concentration were the set of predictors with significant relevance during tissue discrimination. Tissue metabolism, physiology of the organisms and environmental factors may be related to the differences in trace elements and oxidative stress indicators found in muscle, liver and kidney of the mako shark.
KW - Antioxidants
KW - Ecotoxicology
KW - Mako shark
KW - Metals
KW - Oxidative damage
UR - http://www.scopus.com/inward/record.url?scp=84891623145&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2013.03.006
DO - 10.1016/j.cbpa.2013.03.006
M3 - Artículo
SN - 1095-6433
VL - 165
SP - 508
EP - 514
JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
IS - 4
ER -