TY - JOUR
T1 - Oxidative damage in gills and liver in Nile tilapia (Oreochromis niloticus) exposed to diazinon
AU - Toledo-Ibarra, G. A.
AU - Díaz Resendiz, K. J.G.
AU - Ventura-Ramón, G. H.
AU - González-Jaime, F.
AU - Vega-López, A.
AU - Becerril-Villanueva, E.
AU - Pavón, L.
AU - Girón-Pérez, M. I.
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Agricultural activity demands the use of pesticides for plague control and extermination. In that matter, diazinon is one of the most widely used organophosphorus pesticides (OPs). Despite its benefits, the use of OPs in agricultural activities can also have negative effects since the excessive use of these substances can represent a major contamination problem for water bodies and organisms that inhabit them. The aim of this paper was to evaluate oxidative damage in lipids and proteins of Nile tilapia (Oreochromis niloticus) exposed acutely to diazinon (0.97, 1.95 and 3.95 ppm) for 12 or 24 h. The evaluation of oxidative damage was determined by quantifying lipid hydroperoxides (Fox method) and oxidized proteins (DNPH method). The data from this study suggest that diazinon induces a concentration-dependent oxidative damage in proteins, but not lipids, of the liver and gills of Nile tilapia. Furthermore, the treatment leads to a decrease in the concentration of total proteins, which can have serious consequences in cell physiology and fish development.
AB - Agricultural activity demands the use of pesticides for plague control and extermination. In that matter, diazinon is one of the most widely used organophosphorus pesticides (OPs). Despite its benefits, the use of OPs in agricultural activities can also have negative effects since the excessive use of these substances can represent a major contamination problem for water bodies and organisms that inhabit them. The aim of this paper was to evaluate oxidative damage in lipids and proteins of Nile tilapia (Oreochromis niloticus) exposed acutely to diazinon (0.97, 1.95 and 3.95 ppm) for 12 or 24 h. The evaluation of oxidative damage was determined by quantifying lipid hydroperoxides (Fox method) and oxidized proteins (DNPH method). The data from this study suggest that diazinon induces a concentration-dependent oxidative damage in proteins, but not lipids, of the liver and gills of Nile tilapia. Furthermore, the treatment leads to a decrease in the concentration of total proteins, which can have serious consequences in cell physiology and fish development.
KW - Diazinon
KW - Gills
KW - Liver
KW - Nile tilapia
KW - Organophosphorus pesticide
KW - Oxidative damage
UR - http://www.scopus.com/inward/record.url?scp=84969524430&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2016.05.007
DO - 10.1016/j.cbpa.2016.05.007
M3 - Artículo
C2 - 27174646
AN - SCOPUS:84969524430
SN - 1095-6433
VL - 200
SP - 3
EP - 8
JO - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
ER -