Overexpression of AtGRDP2 gene in common bean hairy roots generates vigorous plants with enhanced salt tolerance

María Azucena Ortega-Amaro, Margarita Rodríguez-Kessler, Aída Araceli Rodríguez-Hernández, Alicia Becerra-Flora, Sergio Rosales-Mendoza, Juan Francisco Jiménez-Bremont

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Proteins with glycine-rich repeats have been identified in plants, mammalians, fungi, and bacteria. Plant glycine-rich proteins have been associated to stress response. Previously, we reported that the Arabidopsis thalianaAtGRDP2 gene, which encodes a protein with a glycine-rich domain, plays a role in growth and development of A. thaliana and Lactuca sativa. In this study, we generated composite Phaseolus vulgaris plants that overexpress the AtGRDP2 gene in hairy roots generated by Agrobacterium rhizogenes. We observed that hairy roots harboring the AtGRDP2 gene developed more abundant and faster-growing roots than control hairy roots generated with the wild type A. rhizogenes. In addition, composite common bean plants overexpressing the AtGRDP2 gene in roots were more tolerant to salt stress showing increments in their fresh and dry weight. Our data further support the role of plant GRDP genes in development and stress response.

Original languageEnglish
Article number66
Pages (from-to)1-6
Number of pages6
JournalActa Physiologiae Plantarum
Volume38
Issue number3
DOIs
StatePublished - 1 Mar 2016

Fingerprint

Salt-Tolerance
gene overexpression
salt tolerance
beans
Glycine
Phaseolus
Rhizobium rhizogenes
Genes
genes
stress response
Plant Genes
Lettuce
Agrobacterium
Proteins
Growth and Development
Arabidopsis
proteins
Lactuca sativa
Fungi
Salts

Keywords

  • AtGRDP2
  • Common bean
  • Development
  • Glycine-rich domain protein
  • Hairy roots
  • Salt stress

Cite this

Ortega-Amaro, M. A., Rodríguez-Kessler, M., Rodríguez-Hernández, A. A., Becerra-Flora, A., Rosales-Mendoza, S., & Jiménez-Bremont, J. F. (2016). Overexpression of AtGRDP2 gene in common bean hairy roots generates vigorous plants with enhanced salt tolerance. Acta Physiologiae Plantarum, 38(3), 1-6. [66]. https://doi.org/10.1007/s11738-016-2083-0
Ortega-Amaro, María Azucena ; Rodríguez-Kessler, Margarita ; Rodríguez-Hernández, Aída Araceli ; Becerra-Flora, Alicia ; Rosales-Mendoza, Sergio ; Jiménez-Bremont, Juan Francisco. / Overexpression of AtGRDP2 gene in common bean hairy roots generates vigorous plants with enhanced salt tolerance. In: Acta Physiologiae Plantarum. 2016 ; Vol. 38, No. 3. pp. 1-6.
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abstract = "Proteins with glycine-rich repeats have been identified in plants, mammalians, fungi, and bacteria. Plant glycine-rich proteins have been associated to stress response. Previously, we reported that the Arabidopsis thalianaAtGRDP2 gene, which encodes a protein with a glycine-rich domain, plays a role in growth and development of A. thaliana and Lactuca sativa. In this study, we generated composite Phaseolus vulgaris plants that overexpress the AtGRDP2 gene in hairy roots generated by Agrobacterium rhizogenes. We observed that hairy roots harboring the AtGRDP2 gene developed more abundant and faster-growing roots than control hairy roots generated with the wild type A. rhizogenes. In addition, composite common bean plants overexpressing the AtGRDP2 gene in roots were more tolerant to salt stress showing increments in their fresh and dry weight. Our data further support the role of plant GRDP genes in development and stress response.",
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Ortega-Amaro, MA, Rodríguez-Kessler, M, Rodríguez-Hernández, AA, Becerra-Flora, A, Rosales-Mendoza, S & Jiménez-Bremont, JF 2016, 'Overexpression of AtGRDP2 gene in common bean hairy roots generates vigorous plants with enhanced salt tolerance', Acta Physiologiae Plantarum, vol. 38, no. 3, 66, pp. 1-6. https://doi.org/10.1007/s11738-016-2083-0

Overexpression of AtGRDP2 gene in common bean hairy roots generates vigorous plants with enhanced salt tolerance. / Ortega-Amaro, María Azucena; Rodríguez-Kessler, Margarita; Rodríguez-Hernández, Aída Araceli; Becerra-Flora, Alicia; Rosales-Mendoza, Sergio; Jiménez-Bremont, Juan Francisco.

In: Acta Physiologiae Plantarum, Vol. 38, No. 3, 66, 01.03.2016, p. 1-6.

Research output: Contribution to journalArticle

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T1 - Overexpression of AtGRDP2 gene in common bean hairy roots generates vigorous plants with enhanced salt tolerance

AU - Ortega-Amaro, María Azucena

AU - Rodríguez-Kessler, Margarita

AU - Rodríguez-Hernández, Aída Araceli

AU - Becerra-Flora, Alicia

AU - Rosales-Mendoza, Sergio

AU - Jiménez-Bremont, Juan Francisco

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Proteins with glycine-rich repeats have been identified in plants, mammalians, fungi, and bacteria. Plant glycine-rich proteins have been associated to stress response. Previously, we reported that the Arabidopsis thalianaAtGRDP2 gene, which encodes a protein with a glycine-rich domain, plays a role in growth and development of A. thaliana and Lactuca sativa. In this study, we generated composite Phaseolus vulgaris plants that overexpress the AtGRDP2 gene in hairy roots generated by Agrobacterium rhizogenes. We observed that hairy roots harboring the AtGRDP2 gene developed more abundant and faster-growing roots than control hairy roots generated with the wild type A. rhizogenes. In addition, composite common bean plants overexpressing the AtGRDP2 gene in roots were more tolerant to salt stress showing increments in their fresh and dry weight. Our data further support the role of plant GRDP genes in development and stress response.

AB - Proteins with glycine-rich repeats have been identified in plants, mammalians, fungi, and bacteria. Plant glycine-rich proteins have been associated to stress response. Previously, we reported that the Arabidopsis thalianaAtGRDP2 gene, which encodes a protein with a glycine-rich domain, plays a role in growth and development of A. thaliana and Lactuca sativa. In this study, we generated composite Phaseolus vulgaris plants that overexpress the AtGRDP2 gene in hairy roots generated by Agrobacterium rhizogenes. We observed that hairy roots harboring the AtGRDP2 gene developed more abundant and faster-growing roots than control hairy roots generated with the wild type A. rhizogenes. In addition, composite common bean plants overexpressing the AtGRDP2 gene in roots were more tolerant to salt stress showing increments in their fresh and dry weight. Our data further support the role of plant GRDP genes in development and stress response.

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