TY - JOUR
T1 - Optimization of surfactants formulations to stabilise proteases and amylases
AU - Vicaria, José M.
AU - Herrera-Márquez, Otilia
AU - Serrano-Haro, Mireya
AU - Vidal, Anaïs
AU - Jurado, Encarnación
AU - Jiménez-Pérez, José L.
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/10/12
Y1 - 2022/10/12
N2 - The stability of amylase and protease with linear alkylbenzene sulphonate, ethoxylated fatty alcohol, alkylpolyglucoside, and silica microparticles was analysed. Protease showed first order deactivation finding the highest stability (deactivation energy 62.9 Kcal/mol) with linear alkylbenzene sulfonate/ethoxylated fatty alcohol/silica microparticles (10/10/1.5% w/w). Other surfactant formulations with ethoxylated fatty alcohol (20% w/w), alkylbenzene sulfonate/ethoxylated fatty alcohol (10/10% w/w) or linear alkylbenzene sulfonate/ethoxylated fatty alcohol/alkylpolyglucoside (10/5/5% w/w) also stabilised the protease. Linear alkylbenzene sulfonate deactivated the amylase. Linear alkylbenzene sulfonate/ethoxylated fatty alcohol/ alkylpolyglucoside (10/5/5% w/w) and linear alkylbenzene sulfonate/ethoxylated fatty alcohol/silica microparticles (10/10/1.5% w/w) minimised the amylase destabilisation. These formulations also showed high detergency. Alkylbenzene sulfonate promoted protein unfolding and reduced the enzymatic activity, and the non-ionic surfactants stabilised the enzymes, possibly because they interact harmlessly with the enzymes and avoid interaction with the anionic surfactants at the same binding sites. Optimised surfactant formulations were suggested for detergent formulation with amylase and protease.
AB - The stability of amylase and protease with linear alkylbenzene sulphonate, ethoxylated fatty alcohol, alkylpolyglucoside, and silica microparticles was analysed. Protease showed first order deactivation finding the highest stability (deactivation energy 62.9 Kcal/mol) with linear alkylbenzene sulfonate/ethoxylated fatty alcohol/silica microparticles (10/10/1.5% w/w). Other surfactant formulations with ethoxylated fatty alcohol (20% w/w), alkylbenzene sulfonate/ethoxylated fatty alcohol (10/10% w/w) or linear alkylbenzene sulfonate/ethoxylated fatty alcohol/alkylpolyglucoside (10/5/5% w/w) also stabilised the protease. Linear alkylbenzene sulfonate deactivated the amylase. Linear alkylbenzene sulfonate/ethoxylated fatty alcohol/ alkylpolyglucoside (10/5/5% w/w) and linear alkylbenzene sulfonate/ethoxylated fatty alcohol/silica microparticles (10/10/1.5% w/w) minimised the amylase destabilisation. These formulations also showed high detergency. Alkylbenzene sulfonate promoted protein unfolding and reduced the enzymatic activity, and the non-ionic surfactants stabilised the enzymes, possibly because they interact harmlessly with the enzymes and avoid interaction with the anionic surfactants at the same binding sites. Optimised surfactant formulations were suggested for detergent formulation with amylase and protease.
KW - Amylase
KW - Cleaning efficiency
KW - Protease
KW - Stability
KW - Surfactants
UR - http://www.scopus.com/inward/record.url?scp=85133834438&partnerID=8YFLogxK
U2 - 10.1016/j.ces.2022.117858
DO - 10.1016/j.ces.2022.117858
M3 - Artículo
AN - SCOPUS:85133834438
SN - 0009-2509
VL - 260
JO - Chemical Engineering Science
JF - Chemical Engineering Science
M1 - 117858
ER -