TY - GEN
T1 - Optical techniques coupled to ohmic heating to study heat-induced changes in biological systems
AU - Vázquez-Landaverde, Pedro A.
AU - Sánchez, Eduardo Morales
AU - Huerta-Ruelas, Jorge A.
PY - 2007
Y1 - 2007
N2 - Solutions of bovine serum albumin protein were heated from 30 to 85°C in a temperature-controlled optical cell equipped with air-convective and ohmic heating systems. A polarized laser beam passed through a photo-elastic modulator was used to measure system changes in optical rotation. Results using both the air-convective and the ohmic heating systems showed that when the protein system was heated, an increase in absolute value of optical rotation occurred close to the temperature of denaturation of the protein. Both heating systems were compared, evaluating data points spread and variation in calculated temperature of denaturation for the replicates. Although no improvement in these parameters was obtained with ohmic heating when compared to those obtained with the air-convective system, the optical change related to protein denaturation was more clearly observed. Because ohmic heating strongly interacts with the sample under study, samples with low polarity and not susceptible to electrolysis should be used to avoid electrical effects; regardless, ohmic heating coupled to optical systems could also be a useful tool for the evaluation of interaction of electric field with biological systems optically active.
AB - Solutions of bovine serum albumin protein were heated from 30 to 85°C in a temperature-controlled optical cell equipped with air-convective and ohmic heating systems. A polarized laser beam passed through a photo-elastic modulator was used to measure system changes in optical rotation. Results using both the air-convective and the ohmic heating systems showed that when the protein system was heated, an increase in absolute value of optical rotation occurred close to the temperature of denaturation of the protein. Both heating systems were compared, evaluating data points spread and variation in calculated temperature of denaturation for the replicates. Although no improvement in these parameters was obtained with ohmic heating when compared to those obtained with the air-convective system, the optical change related to protein denaturation was more clearly observed. Because ohmic heating strongly interacts with the sample under study, samples with low polarity and not susceptible to electrolysis should be used to avoid electrical effects; regardless, ohmic heating coupled to optical systems could also be a useful tool for the evaluation of interaction of electric field with biological systems optically active.
KW - Bovine serum albumin
KW - Ohmic heating
KW - Optical rotation
KW - Protein denaturation
UR - http://www.scopus.com/inward/record.url?scp=37649003828&partnerID=8YFLogxK
U2 - 10.1117/12.742352
DO - 10.1117/12.742352
M3 - Contribución a la conferencia
AN - SCOPUS:37649003828
SN - 0819465321
SN - 9780819465320
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Sixth Symposium Optics in Industry
T2 - 6th Symposium Optics in Industry
Y2 - 8 March 2007 through 9 March 2007
ER -