TY - JOUR
T1 - On-line monitoring of industrial interest Bacillus fermentations, using impedance spectroscopy
AU - Díaz Pacheco, Adrián
AU - Dinorín-Téllez-Girón, Jabel
AU - Martínez Montes, Francisco Javier
AU - Martínez Tolibia, Shirlley E.
AU - López y López, Víctor Eric
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/1/10
Y1 - 2022/1/10
N2 - Impedance spectroscopy is a technique used to characterize electrochemical systems, increasing its applicability as well to monitor cell cultures. During their growth, Bacillus species have different phases which involve the production and consumption of different metabolites, culminating in the cell differentiation process that allows the generation of bacterial spores. In order to use impedance spectroscopy as a tool to monitor industrial interest Bacillus cultures, we conducted batch fermentations of Bacillus species such as B. subtilis, B. amyloliquefaciens, and B. licheniformis coupled with this technique. Each fermentation was characterized by the scanning of 50 frequencies between 0.5 and 5 MHz every 30 min. Pearson's correlation between impedance and phase angle profiles (obtained from each frequency scanned) with the kinetic profiles of each strain allowed the selection of fixed frequencies of 0.5, 1.143, and 1.878 MHz to follow-up of the fermentations of B. subtilis, B. amyloliquefaciens and B. licheniformis, respectively. Dielectric profiles of impedance, phase angle, reactance, and resistance obtained at the fixed frequency showed consistent changes with exponential, transition, and spore release phases.
AB - Impedance spectroscopy is a technique used to characterize electrochemical systems, increasing its applicability as well to monitor cell cultures. During their growth, Bacillus species have different phases which involve the production and consumption of different metabolites, culminating in the cell differentiation process that allows the generation of bacterial spores. In order to use impedance spectroscopy as a tool to monitor industrial interest Bacillus cultures, we conducted batch fermentations of Bacillus species such as B. subtilis, B. amyloliquefaciens, and B. licheniformis coupled with this technique. Each fermentation was characterized by the scanning of 50 frequencies between 0.5 and 5 MHz every 30 min. Pearson's correlation between impedance and phase angle profiles (obtained from each frequency scanned) with the kinetic profiles of each strain allowed the selection of fixed frequencies of 0.5, 1.143, and 1.878 MHz to follow-up of the fermentations of B. subtilis, B. amyloliquefaciens and B. licheniformis, respectively. Dielectric profiles of impedance, phase angle, reactance, and resistance obtained at the fixed frequency showed consistent changes with exponential, transition, and spore release phases.
KW - B. amyloliquefaciens
KW - B. licheniformis
KW - B. subtilis
KW - Impedance spectroscopy
KW - in situ
UR - http://www.scopus.com/inward/record.url?scp=85119965119&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2021.11.005
DO - 10.1016/j.jbiotec.2021.11.005
M3 - Artículo
C2 - 34826536
AN - SCOPUS:85119965119
SN - 0168-1656
VL - 343
SP - 52
EP - 61
JO - Journal of Biotechnology
JF - Journal of Biotechnology
ER -